Introduction to TopSENSI ® Human FluA/B RT-qPCR Kit
Influenza virus is a respiratory pathogen that commonly causes acute febrile illness known as influenza. Belonging to the Orthomyxoviridae family, influenza viruses can infect multiple hosts including humans, swine, and birds. Human influenza viruses are classified into three types—A, B, and C—based primarily on antigenic differences in their NP and M proteins. Among these, Influenza A and B viruses are the most common causes of seasonal influenza outbreaks in humans.
Influenza B Virus (IBV) typically circulates later in the season and accounts for approximately 15–30% of total influenza cases. Although the severity of illness caused by Influenza A and B is comparable, IBV does not cause pandemics. In contrast, Influenza A Virus (IAV) is often responsible for seasonal epidemics and pandemics due to frequent genetic mutations and reassortment among subtypes.
TopSENSI ® Human FluA/B RT-qPCR Kit is designed to detect the presence of IAV (M gene) and IBV (NP gene) associated with human seasonal influenza using the RT‑qPCR technique. The kit offers several outstanding advantages such as a simple and rapid workflow, high sensitivity, and high specificity. In particular, the kit employs an endogenous internal control (Human RNase P gene) to effectively monitor the processes of sample collection, RNA extraction, and RT‑qPCR.
HIGHLIGHTS
- Simple process easy manipulation
- PCR execution time is quick, only about 2 hours
- High specificity and sensitivity
- Taqman probe technology

TopSENSI ® Human FluA/B RT-qPCR Kit (SQH-015)
SPECIFICATIONS
| Targets | Influenza virus (IAV - M gene and IBV - NP gene) |
| Extraction sample type | Nasal, throat, nasopharyngeal, and bronchial specimens |
| PCR sample volume | 10 µL |
| Fluorescent channels | FAM: IAV – M gene HEX: IBV – NP gene Cy5: Human RNase P gene (Internal control) |
| Technology | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Sensitivity | IAV: 30,00 copies/reaction IBV: 35,00 copies/reaction |
| Analytical Specificity | Kit only detects IAV and IBV |
| Storage | 12 months at -20oC |
| Kit components | HuFluA/B RT-qPCR Mix, Negative control, Positive control, PCR tube |
Recommended extraction kit
| Silica column extraction | TopPURE® Serum Viral RNA Extraction Kit |
| TopPURE ® Serum Viral DNA/RNA Co-Extraction Kit | |
| TopPURE® Viral DNA/RNA Extraction Kit | |
| Automatic magbead extraction | TopPURE® Maga Serum DNA/RNA Co-Extraction Kit |
| TopPURE® Maga Viral DNA/RNA Extraction Kit |
Read more: Instructional Video for Qualitative Real-time PCR Kit




Reviews
There are no reviews yet.