TopPURE® FFPE DNA EXTRACTION KIT (HE-005)

INTRODUCTION TO TopPURE® FFPE DNA EXTRACTION KIT (HE-005)

FFPE refers to formalin-fixed, paraffin-embedded (FFPE) tissue, commonly used in cancer biopsy samples to preserve tissue structure for long-term storage. This method provides a valuable resource for retrospective cancer research, histopathology, and molecular genetics. Extracting DNA from FFPE tissues is a crucial initial step in accurately detecting genetic mutations, quantifying oncogene expression levels, and performing analyses such as gene sequencing and real-time PCR.

TopPURE® FFPE DNA Extraction Kit is developed using silica column technology, meeting ISO 9001 and ISO 13485 standards, ensuring high-quality DNA recovery from FFPE tissue samples. The kit is designed for room temperature storage and offers an extended shelf life of up to 24 months, enhancing cost efficiency and convenience for users.

TopPURE® FFPE DNA Extraction Kit (HE-005)

HIGHLIGHTS

• Safe: Kit does not contain hazardous chemicals
• The quality of the resulting DNA has a high integrity, helping to increase PCR amplification of large segments
• Simple and effecitive procedure
• Efficient: DNA is extracted with high yield and purity
• Completed solution: include Paraffin removal step and DNA extraction
• Convenient: Provides a complete set of materials and reagents, eliminating the need for additional external chemicals

SPECIFICATIONS

• Sample type: FFPE
• Input sample quantity: < 8 tissue sections, with a thickness of 5-10 µm
• Extraction time: 3 hour for 10 samples
• Elution volume: 50 µL DNA
• Purity: A260/A280 ~ 1.7-2.2
• Storage condition: temperature 15-35°C
• Expiration date: 24 months from the manufacturing date

^(*) Add ethanol to WB1 Buffer and WB2 Buffer before first use, following the instructions on the bottle label.

^(**) Proteinase K is reconstituted with Dissolve Buffer before use, following the instructions on the tube label. After reconstitution, the tube should be stored at 2-8°C.

Read more:

Other column extraction kit

Instructional Video for ABT Kit