TAE BUFFER 1X

Specifications

TAE Buffer 1X (Tris-Acetate-EDTA) is a commonly used buffer for DNA and RNA electrophoresis on agarose gels and polyacrylamide gels. Can be used for electrophoresis of both genomes and supercoiled DNA. Used as buffer for electrophoresis and gel preparation. Should be used for electrophoresis of RNA and DNA fragments larger than 1500 bp, genomes, supercoiled DNA. The product is in the form of a homogeneous, transparent solution. Store at room temperature.

Manual instruction

• Weigh the appropriate amount of agarose (depending on the gel percentage) into a bottle of appropriate volume.
• Add appropriate amount of TAE 1X buffer and shake well.
• Boil the mixture in the microwave until the agarose is completely dissolved.
• Cool the gel to 50-55 ̊C, pour the gel into the mold and insert the comb. Be careful not to let air bubbles appear. If air bubbles appear, carefully push them to the sides with the tip of a pipette.
• Place the newly poured gel at a temperature of 4 ̊C for about 10-15 minutes (in case of urgent need) or leave it at room temperature for about 20-30 minutes, until the gel is completely solidified.
• When the gel cools, place the gel mold in the electrophoresis chamber, remove the comb and add 1X TAE buffer to the gel tray so that the buffer is 2 mm above the top of the gel.
• Load samples proportionally: Load samples proportionally: 5μL PCR product + 1μL 6X GelRed Loading Buffer into each well. The DNA ladder uses a ratio of 5 μL of DNA ladder and 1μL 6X GelRed Loading Buffer
• Electrophoresis at 85 V for 30 minutes or until the yellow indicator line reaches the end of the gel.
• Can use TopPURE ® PCR/GEL DNA PURIFICATION KIT  to purify products after electrophoresis using the silica column method.