GELRED 3X IN WATER

Specifications

GelRed 3X in water is an ultrasensitive, highly stable, and environmentally safe fluorescent nucleic acid staining solution designed to replace ethidium bromide (EtBr), a highly toxic compound. GelRed 3X in water is used for staining double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), or RNA in agarose or polyacrylamide gels. GelRed and EtBr exhibit nearly identical spectral properties, allowing GelRed to be directly substituted for EtBr without requiring modifications to existing imaging systems.

GelRed 3X in water – 100mL Bottle

Manual instruction

Method 1: Post-Electrophoresis Gel Staining with GelRed 3X in Water

• Step 1: Cast the gel by pouring molten agarose into a mold with a comb in place. Allow the gel to solidify at room temperature.
• Step 2: Load samples and perform electrophoresis according to standard procedures.
• Step 3: Prepare the staining solution by diluting GelRed 3X in water to a final concentration of 1X using H₂O (Example: Mix 20 mL of GelRed 3X in water with 40 mL of H₂O to obtain 60 mL of GelRed 1X).
• Step 4: Submerge the gel in the GelRed 1X solution, ensuring the liquid covers the gel by at least 2 mm. Incubate for 10–15 minutes in the dark.
• Step 5: Visualize the stained gel using a standard UV transilluminator (302 nm or 312 nm) and capture images using a UV light filter.

Method 2: Pre-Casting Gel with GelRed 3X in Water

• Step 1: Dilute GelRed 3X in water to 1X using concentrated electrophoresis buffer. Example: Mix 20 mL of GelRed 3X in water with 6 mL of 10X TBE buffer and 34 mL of H₂O to obtain 60 mL of 1X GelRed in 1X TBE).
• Step 2: Add the required amount of agarose to the GelRed 1X solution, mix thoroughly, and heat until fully dissolved.
• Step 3: Pour the molten gel into a mold with a comb in place and allow it to solidify at room temperature.

Note:

- Unused gel solution can be stored and reheated for future use.

- Molten gel (at ~50°C) should be used within 1–2 days.

- Cast gels can be stored at room temperature in the dark for up to one week.

- Avoid storing cast gels at 4°C, as this may cause dye precipitation and reduce staining efficiency.

• Step 4: Load samples and perform electrophoresis as usual.
• Step 5: Visualize the stained gel using a standard UV transilluminator (302 nm or 312 nm) and capture images using a UV light filter.

Recommendations

- Design protocols tailored to sample type and intended application.

- Use appropriate voltage and run time for each electrophoresis chamber.

- Ensure correct electrode placement during electrophoresis.

- Regularly clean and UV-sterilize the electrophoresis area. Consider using TopCLEAN ® DNA/RNA AWAY to eliminate residual DNA/RNA and prevent cross-contamination.
- Avoid direct exposure to GelRed via inhalation, skin contact, eye contact, or ingestion.

Read more:

• Other Bio-reagents
• Manual instruction