CloneSmart® Seamless DNA Assembly Kit – Cloning Kit
INTRODUCTION
CloneSmart® Seamless DNA Assembly Kit is a one‑step cloning solution based on the principle of joining homologous sequences (15–25 bp) between DNA fragments.

CloneSmart® Seamless DNA Assembly Kit
HIGHLIGHTS
- Does not require restriction enzymes or DNA ligase.
- Eliminates complex processing steps of traditional cloning methods.
- Enhances flexibility in experimental design.
- Efficient assembly of 1–3 DNA fragments ≥100 bp.
- Simple and rapid workflow: incubation at 30 °C for 10 minutes.
SPECIFICATIONS
- Components: Exo Enzyme Mix, 3X Exo Buffer, pUC57-Kan Control Vector (Linearized), Control Insert, Nuclease-free Water
- Storage conditions: –20 °C
- Expiration date: 12 months from the date of manufacture
- Packaging: 10 tests/box
- Product code: PC‑01
Read more:
User Manual: CloneSmart® Seamless DNA Assembly Kit








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