ABT® HEMATOXYLIN STAIN SOLUTION (DD-095)

Introducing ABT® HEMATOXYLIN STAIN SOLUTION

Intended use: ABT® Hematoxylin Stain Solution is designed for nuclear staining and differentiation of cell types in cytology testing using the Papanicolaou staining method.

Product description:

• ABT® Hematoxylin Stain Solution is a nuclear stain for cells, provided in a ready-to-use form.
• ABT® Hematoxylin Stain Solution is optimal for staining and differentiating various cell types in cytological examinations using the Papanicolaou staining method.

Specification: 

• Appearance: The solution is a uniform dark red liquid without suspended particles or sediment. It has a characteristic product smell, without any foreign odors.
• Staining capabilities: Cells stained with this solution exhibit blue-purple nuclei.
• Components: Ethylene glycol, Hematoxylin, Sodium iodate, Aluminum ammonium sulfate dodecahydrate.
• Packaging: 500mL/Bottle
• Expiration date: 12 months from manufacture
• Storage condition: Store at 15-30℃, away from direct sunlight.

Manual instruction

1. Papanicolaou (PAP) staining chemicals according to the following protocol:

Other equipment not supplied:

• Staining kit
• Microscope slides

Other chemicals not supplied:

• ABT ® Hematoxylin Stain Solution (DD-095)
• ABT ® OG Stain Solution (DD-096)
• Ethanol 96
• Rinse water*

Note:

* Rinse water: Can use tap water with pH ≥ 8, or alkaline bluing solutions such as Scott’s water, 0.05% Ammonia solution, or saturated lithium carbonate.

** Stains: Provided in a ready-to-use form. Simply shake the stain well and pour it into the container with the appropriate volume for use. Store in a tightly closed dark bottle after use.

2. Sample preparation:

• Liquid-based Pap Test:

- The cervical fluid sample will be collected and processed following the procedure outlined in the ABT ® Cell Preservative Solution (DD-104).

- The liquid-based sample is transferred onto a microscope slide using an automated slide preparation machine and the corresponding filter cup.

• Traditional Pap Test:

After collection, cervical smear samples are directly smeared onto glass slides.

3. Papanicolaou Staining (PAP) Procedure:

- Step 1: Fix the sample with 96% Ethanol in box 1 for 15 minutes.

- Step 2: Dip the slide five times into the rinsing water in box 2 after fixation.

- Step 3: Stain the nuclei with ABT® Hematoxylin Stain Solution (DD-095) in box 3 for 1 minute.

- Step 4: Dip the slide five times into the rinsing water in box 2 after nuclear staining.

- Step 5: Successively dip the slide into 96% Ethanol in box 4 and 5. Repeat this process five times for each box.

- Step 6: Stain the cytoplasm with ABT® OG Stain Solution (DD-096) in box 6 for 1 minute.

- Step 7: Successively dip the slide into 96% Ethanol in box 7 and 8. Repeat this process five times for each box.

- Step 8: Stain the cytoplasm with ABT® EA Stain Solution (DD-094) in box 9 for 2 minutes.

- Step 9: Successively dip the slide into 96% Ethanol in box 10, 11, and 12. Repeat this process five times for each box.

- Step 10: Allow the slide to dry, apply mineral oil, and observe directly under the microscope.

Read more:

Detailed Instructions for Use.