6X GELRED LOADING BUFFER WITH TRICOLOR

Specifications

6x GelRed Loading Buffer With Tricolor is a sample loading solution containing a high-density agent, tracking dyes, and GelRed fluorescent stain. It includes three distinct tracking dyes—Bromophenol Blue, Xylene Cyanol FF, and Orange G—that enable visual monitoring of DNA migration during electrophoresis. 6x GelRed Loading Buffer With Tricolor is added directly to the sample and loaded into the gel, eliminating the need to incorporate fluorescent DNA stains into the agarose gel during casting.

Manual instruction

• Use distilled water to dilute 50X TAE buffer (or 10X TBE buffer) to 1X TAE (or 1X TBE) before use.
• Weigh the appropriate amount of agarose (depending on desired gel percentage) and place it in a suitable container.
• Add the correct volume of 1X TAE (or 1X TBE) buffer and mix well.
• Boil the mixture in the microwave until the agarose is completely dissolved.
• Cool the gel to a temperature of 50–55°C, then pour it into the casting tray and insert the comb. Take care to avoid the formation of air bubbles; if bubbles appear, gently displace them to the sides using a pipette tip.
• Place the newly poured gel at a temperature of 4°C for about 10-15 minutes (in case of urgent need) or leave it at room temperature for about 20-30 minutes, until the gel is completely solidified.
• Once cooled, place the gel tray into the electrophoresis chamber. Remove the comb and add 1X TAE (or 1X TBE) buffer to the gel tank until the buffer level is about 2 mm above the gel surface.
• Load samples at a ratio of 5 μL PCR product to 1 μL 6x Gelred Loading buffer per well. For the DNA ladder, use 5 μL of ladder mixed with 1 μL of 6X GelRed Loading Buffer.
• Perform electrophoresis at a voltage of 85 V for 30 minutes, or until the yellow tracking dye reaches the end of the gel. The running time and voltage may be adjusted as needed based on experimental requirements.

Caution

• Use appropriate voltage and duration for each electrophoresis chamber.
• Ensure correct electrode placement during electrophoresis.
• Regularly clean and UV-sterilize the electrophoresis room.
• Avoid direct exposure via inhalation, skin contact, eye contact, and ingestion.

Read more:

• Other Bio-reagents
• Manual instruction