Introduction

Vibrio alginolyticus is a Gram-negative bacterium belonging to the Vibrionaceae family, characterized by its motility, facultative anaerobic nature, and typically straight or slightly curved rod-shaped morphology.V. alginolyticus is known to cause red body syndrome in postlarval shrimp. Affected postlarvae exhibit red spots on the base of antennae, head-thorax junction, body, and appendages when infected with Vibrio alginolyticus. Additionally, numerous studies indicate that, along with V. cholera, V. alginolyticus is implicated in causing white feces syndrome in whiteleg shrimp. Therefore, early detection of the presence of this bacterium in aquaculture environments is crucial to mitigate the potential severe consequences it may induce.

TopSPEC® V. alginolyticus qPCR Kit is a testing kit designed to detect the presence of Vibrio alginolyticus in aquatic samples using the Real-time PCR technique. The kit enables the rapid detection of the pathogen with high sensitivity and specificity.

TopSPEC® V. alginolyticus qPCR Kit

Highlights TopSPEC® V.alginolyticus qPCR Kit

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Vibrio alginolyticus – toxR gene
Sample types	DNA after extraction
DNA volume	5 µL
Color channel detection	FAM: detect Vibrio alginolyticus HEX: internal control
Technology	TaqMan probe
PCR time	1.5h
Specificity	Kit only detects V. alginolyticus
Component	Vibrio alginolyticus qPCR mix, negative control, positive control, internal control, PCR tube
Storage	12 months at -20^oC