Specifications

6X GelGreen Loading Buffer With Tricolor is a sensitive, stable, and environmentally safe green fluorescent nucleic acid dye, specially designed for gel staining. GelGreen offers significantly higher sensitivity compared to SYBR Safe. Unlike SYBR dyes—which are known for their instability—GelGreen is highly stable against both hydrolysis and thermal degradation. This buffer is optimally excited at 497 nm and also exhibits a secondary excitation peak at 248 nm. Upon binding to DNA, its fluorescence emission is centered at 524 nm. These spectral properties make 6X GelGreen Loading Buffer With Tricolor compatible with a wide range of gel imaging systems.

6X GelGreen Loading buffer With Tricolor

Manual instruction

Use distilled water to dilute 50X TAE buffer (or 10X TBE buffer) to 1X TAE (or 1X TBE) before use.
Weigh the appropriate amount of agarose (depending on desired gel percentage) and place it in a suitable container.
Add the correct volume of 1X TAE (or 1X TBE) buffer and mix well.
Boil the mixture in the microwave until the agarose is completely dissolved.
Cool the gel to 50-55 ̊C, pour the gel into the mold and insert the comb. Be careful not to let air bubbles appear. If air bubbles appear, carefully push them to the sides with the tip of a pipette.
Place the newly poured gel at a temperature of 4 ̊C for about 10-15 minutes (in case of urgent need) or leave it at room temperature for about 20-30 minutes, until the gel is completely solidified.
Once cooled, place the gel tray into the electrophoresis chamber. Remove the comb and add 1X TAE (or 1X TBE) buffer to the gel tank until the buffer level is about 2 mm above the gel surface.
PCR products loading: Mix 5 μL of PCR product with 1 μL of 6X GelGreen Loading Buffer With Tricolor and load into each well. DNA ladder: Mix 5 μL of DNA ladder with 1 μL of 6X GelGreen Loading Buffer With Tricolor.
Electrophoresis: Run the gel at 85 V for 30 minutes, or adjust voltage and time according to experimental needs.