Introducing TopSPEC® LSNV One-step RT-qPCR Kit
Monodon Slow Growth Syndrome (Monodon Slow Growth Syndrome – MSGS) is one of the major concerns, seriously affecting the shrimp farming industry. Laem Singh Virus (LSNV)was identified as associated with shrimp infected with MSGS. LSNV is an RNA virus about 25 nm in diameter that commonly infects shrimp lymph nodes, gills and nervous tissue. Shrimp infected with LSNV do not show obvious signs of disease but have slow growth, so the farming season often has to be extended, increasing costs. Besides, reduced shrimp size will also reduce productivity and value of farmed shrimp.
TopSPEC® LSNV One-step RT-qPCR Kit is a diagnostic kit designed to detect the LSNV virus causing disease in shrimp using RT-qPCR technology. The kit features a simple and rapid workflow with high sensitivity and specificity, enabling timely diagnosis and minimizing losses caused by the disease.
TopSPEC® LSNV One-step RT-qPCR Kit
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- High specificity and sensitivity
Specification
| Targets | Laem Singh Virus (LSNV) – RdRp gene |
| Input sample | RNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | Cy5 color channel: LSNV detection HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects LSNV |
| Storage | 12 months at -20oC |
| Kit components | LSNV One-step RT- qPCR mix, negative control, positive control, internal control, PCR tube |
Recommended extraction kit
TopPURE® Highpure Viral Extraction Kit (EXS-021)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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