Introduction TopSPEC® ADV Multiplex qPCR Kit
Aujeszky's disease (AD) is a highly infectious and dangerous disease that affects pigs of all ages, with a high mortality rate. The disease is caused by a DNA virus belonging to the Herpesviridae family (PRV). AD causes symptoms such as fever, cough, runny nose, pneumonia, and abortion, as well as fetal death in sows. Currently, the most effective measure to control and minimize the damage caused by AD is whole-herd vaccination.
TopSPEC® ADV Multiplex qPCR Kit is a diagnostic assay designed to detect and differentiate pigs infected with wild-type Aujeszky’s Disease Virus (ADV/PRV), which carries the Glycoprotein B gene, from those vaccinated with inactivated strains lacking the Glycoprotein E gene. Utilizing real-time PCR technology, the kit enables rapid detection of ADV with high sensitivity and specificity.
TopSPEC® ADV Multiplex qPCR Kit
Highlights
- Simple procedure
- PCR time is optimized for 2 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Wild-type ADV (PRV) carrying the Glycoprotein B gene or inactivated vaccine strains lacking the Glycoprotein E gene." |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: detects Glycoprotein B gene, Texas Red: detects Glycoprotein E gene, HEX: internal control (IC) |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detect wild type ADV and vaccine strain |
| Storage | 12 months at -20oC |
| Kit components | ADV multiplex qPCR mix, negative control, positive control, internal control, PCR tube |
| Recommended for extraction kit | ||
| Silica column extraction | TopPURE® Genomic DNA Extraction Kit | |
| TopPURE® Blood DNA Extraction Kit | ||
| TopPURE® Serum Viral DNA/RNA Co-Extraction Kit | ||
Read more: Instructional Video for Qualitative Real-time PCR Kit
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