Introduction
Currently, the situation of meat being counterfeited with chemicals and mixing meats to increase profits in food is increasing. Therefore, distinguishing and detecting meat of the right origin and right type is essential not only in exporting finished meat products but also in certifying vegan food or in health issues (such as fasting). due to meat allergy,...) and in religious cuisine (such as Islam does not use pork, Hinduism does not use beef,...)
TopSPEC® Bovine DNA Detection Kit facilitates the assessment of the presence of bovine DNA (encompassing Bos taurus, Bos indicus, Bison bison and Bos mutus) in food and livestock feed samples using Real-time PCR technology, offering high sensitivity and specificity.
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Bovine DNA is derived from food and livestock feed |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: detection of bovine DNA HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects bovine DNA |
| Storage | 12 months at -20oC |
| Kit components | Bovine qPCR mix, negative control, positive control, internal control, PCR tube |
| Recommended for extraction kit | ||
| Silica column extraction | HI-112 | TopPURE® Genomic DNA Extraction Kit |
| HE-013 | TopPURE® Food DNA Extraction Kit | |
| Automatic magbead extraction | HI-612 | TopPURE® Maga Genomic DNA/RNA Extraction |
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