INTRODUCTION

Food poisoning is caused by improperly prepared or unsafe food. One of the factors contributing to this condition is the bacterium Listeria monocytogenes. Listeria is a Gram-positive bacterium capable of surviving for extended periods in the natural environment and in food. The bacterium enters the body through the digestive tract and can also infect the bloodstream, causing sepsis. Notably, pregnant women who contract the disease can transmit it to the fetus, leading to miscarriage and serious effects on newborns. Therefore, food must be carefully prepared and ensured to be safe and hygienic.

TopSPEC® Listeria monocytogenes qPCR Kit is a screening test kit for Listeria monocytogenes bacteria in food microbiology, aiding in food safety control and ensuring health standards.

TopSPEC® Listeria monocytogenes qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Listeria monocytogenes
Sample types	DNA after extraction
Input sample volume	200 µL
DNA volume	5 µL
Color channel detection	FAM: detect Listeria monocytogenes HEX: internal control
Technology	TaqMan probe
PCR time	1.5h
Specificity	Kit only detects Listeria monocytogenes
Component	Lis qPCR mix, negative control, positive control, internal control (IC), Tube PCR.
Storage	12 months at -20^oC