Introduction TopSPEC® Ehrlichia qPCR Kit

Ehrlichiosis is a disease caused by Ehrlichia spp. bacteria, primarily transmitted through dog ticks. Ehrlichia invades white blood cells and adversely affects canine health. The disease progresses through three stages: acute, subclinical, and chronic. Common clinical signs include fever, hematologic abnormalities, and general deterioration of the animal’s health. Although the mortality rate is relatively low, the disease can lead to long-term complications in dogs and, less commonly, in cats.

TopSPEC® Ehrlichia qPCR Kit s a diagnostic assay designed to detect the presence of Ehrlichia spp. in canine and feline samples using qPCR technology targeting the citrate synthase (gltA) gene. Early detection of the pathogen is essential for minimizing disease-related losses and enabling timely and effective preventive measures.

TopSPEC® Ehrlichia qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Ehrlichia – citrate synthase (gltA) gene
Sample types	Serum samples, whole blood samples
Input sample volume	200 µL
DNA volume	5 µL
Color channel detection	HEX: citrate synthase (gltA) gene of Ehrlichia Cy5: internal control
Technology	TaqMan probe
PCR time	1.5h
Specificity	Kit only detects Ehrlichia
Component	Ehrlichia qPCR mix, negative control, positive control, internal control (IC), PCR tube
Storage	12 months at -20^oC