INTRODUCTION
Stunting disease in shrimp appears at almost all stages of development, from mysis to fingerlings and even adult shrimp. The cause of the disease is due to parasitism on hepatopancreatic epithelial cells and intestinal cells of Penaeus Monodon Baculovirus (MBV). MBV destroys hepatopancreatic tissue and digestive tract membranes, causing growth retardation of farmed shrimp. The disease only spreads horizontally, the infection rate in farmed shrimp ranges from 20-100%, however, in the early stages of infection there are often no signs, so screening and early detection of the disease is essential to avoid causing disease. damages.
TopSPEC® MBV qPCR Kit is a test kit that detects the presence of Penaeus Monodon Baculovirus(MBV) in culture environments. Early detection of pathogens helps save costs caused by MBV.
TopSPEC® MBV qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Penaeus Monodon Baculovirus (MBV) – Hypothetical protein gene |
| Sample types | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect MBV HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detect MBV |
| Component | MBV qPCR mix, negative control, positive control, internal control(IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit









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