Introduction

Necrotizing hepatopancreatitis, also known as black spot disease, is caused by Necrotizing hepatopancreatitis bacterium (NHPB). The disease occurs when the pond environment, especially the bottom of the pond, is of poor quality. The mortality rate is up to 95% within 15-30 days of getting sick. The infected shrimps show signs of lethargy, loss of appetite, slow growth, having tiny black spots or large patches and other symptoms. Without timely preventive measures, the situation will get worse. Early detection is the best measure.

TopSPEC® NHPB qPCR Kit is a testing kit designed to detect the presence of Necrotizing Hepatopancreatitis Bacterium (NHPB) in aquaculture environments. Early detection of the pathogen helps minimize economic losses caused by NHPB.

TopSPEC® NHPB qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Necrotizing Hepatopancreatitis Bacterium (NHPB) – 16S ribosomal RNA gene
Sample types	DNA after extraction
Input sample volume	200 µL
DNA volume	5 µL
Color channel detection	FAM: detect NHPB HEX: internal control
Technology	TaqMan probe
PCR time	1.5h
Specificity	Only NHPB is detected
Component	NHPB qPCR mix, negative control, positive control, internal control(IC), Tube PCR.
Storage	12 months at -20^oC