Introducing TopSPEC® IMNV One-step RT-qPCR Kit

Myofascial necrosis disease (IMV) causes significant mortality in the shrimps such as white shrimp (Penaeus vannamei) and black tiger shrimp (P. monodon), brown tiger shrimp (Penaeus esculentus), white shrimp (P. merguiensis) with high spread rate both horizontally and vertically. The tissue carrying the disease is usually skeletal muscle and, less commonly, cardiac muscle. The mortality rate of infected shrimp is up to 70%. Disease can occur at any stage of shrimp development, so early screening and detection is very important.

TopSPEC® IMNV One-step RT-qPCR Kit is a testing kit designed to detect the presence of Infectious Myonecrosis Virus (IMNV) in aquaculture environments. Early detection of the pathogen helps minimize economic losses caused by IMNV.

TopSPEC® IMNV One-step RT-qPCR Kit

Highlights

Simple procedure
PCR time was optimized for 2 hours
Various input sample types
High specificity and sensitivity

Specification

Targets	Infectious Myonecrosis Virus (IMNV) – capsid protein gene
Sample types	RNA after extraction
Input sample volume	200 µL
RNA volume	5 µL
Color channel detection	FAM: detect IMNV HEX: internal control
Technology	TaqMan probe; One-step RT-qPCR
PCR time	1 hour 45 minutes
Specificity	Only IMNV is detected
Component	IMNV One-step RT-qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube.
Storage	12 months at -20^oC