INTRODUCTION TopPURE® VIRAL DNA/RNA EXTRACTION KIT (HK-008)
TopPURE® VIRAL DNA/RNA EXTRACTION KIT is designed for the simultaneous extraction and collection of high-purity DNA/RNA from a wide range of input sample matrices, including cultured cells, bacteria, suspension samples, surface swabs, medical swab samples, throat/nasal swabs, serum, plasma, and whole blood samples from mammals.
The kit is designed and manufactured by ABT in compliance with ISO 9001 and ISO 13485 standards, ensuring the extraction of high-quality DNA/RNA using silica column technology. The kit utilizes VBL lysis buffer, enabling efficient lysis across diverse sample matrices. The nucleic acid obtained after extraction is ready for various downstream applications, including Real-time PCR, sequencing, Southern blotting, and SNP analysis. Additionally, the kit has been optimized for storage at room temperature with an extended shelf life of up to 24 months, providing cost efficiency and user convenience. Furthermore, it is ethanol-free, making it suitable for transportation under regulations restricting the shipment of flammable substances, such as air transport.
TopPURE® Viral DNA/RNA Extraction Kit (HK-008)
HIGHLIGHTS
- The kit can extract a variety of different sample matrix
- High quality and purity
- No organic solvent or toxic reagent (Phenol, Chloroform)
- Convenient: Provide a complete all chemical without needing to add chemicals or materials outside
- The kit includes Ethanol for mixing Wash Buffer 1 and Wash Buffer 2
SPECIFICATIONS
- Input samples: Cultured cells, bacteria, suspension samples, surface swabs, medical swab samples, throat/nasal swabs, serum, plasma, and whole blood samples from mammals.
- Input sample volume: 200 µL
- Elution volume: 50 µL DNA/RNA
- Extraction time: 30 minutes /10 samples
- High purity: A260/A280 ≥ 1.7
- Storage condition: temperature 15-35°C
- Expiration date: 24 months from the manufacturing date
| Component | Storage |
| VBL Buffer | Temperature 15-35°C |
| WB1 Buffer (*) | |
| WB2 Buffer (*) | |
| EB Buffer | |
| Proteinase K (**) | |
| Dissolve Buffer | |
| PBS Buffer | |
| Silica column | |
| Tube 1.5mL |
(*) Add ethanol to WB1 Buffer and WB2 Buffer before first use, following the instructions on the bottle label. The product does not include ethanol.
(**) Proteinase K is reconstituted with Dissolve Buffer before use, following the instructions on the tube label. After reconstitution, the tube should be stored at 2-8°C.
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