Introduction TopSPEC® E.coli STEC qPCR Kit

Escherichia coli are gram-negative bacteria that are commensal microorganisms in the human gut. However, certain strains of E. coli can cause intestinal and extraintestinal diseases in humans, such as urinary tract infections (UTI), septicemia, and meningitis. Shiga toxin-producing E. coli (STEC) are considered enterohaemorrhagic E. coli (EHEC) or verocytotoxin-producing E. coli (VTEC). They cause diarrhea as well as more severe diseases in humans, such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). The cause of these syndromes is identified as the Shiga toxins (Stx1 and Stx2) produced by the serotype O157:H7 strain.

The TopSPEC® E.coli STEC qPCR Kit is a screening kit used to detect the presence of Shiga toxin-producing Escherichia coli (STEC) using qPCR technology based on the presence of the Stx1 gene, Stx2 gene, and eaeA gene (also known as eae).

TopSPEC® E.coli STEC qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	STEC (Stx1 gene, Stx2 gene and eaeA gene)
Sample types	DNA after extraction
DNA volume	5 µL
Color channel detection	FAM: Stx1 gene TexasRed: Stx2 gene HEX: eaeA gene Cy5: internal control (IC)
Technology	TaqMan probe
PCR time	1.5h
Specificity	Kit only detects STEC
Component	STEC qPCR mix, negative control, positive control, internal control, PCR tube
Storage	12 months at -20^oC