TopSPEC® WSSV/EMS PCR Kit (SPS-003)

About TopSPEC® WSSV/EMS PCR Kit

White spot disease and microsporidian disease are significant challenges in shrimp aquaculture.

– White spot disease caused by the White Spot Syndrome Virus (WSSV). This is a double-stranded DNA virus belonging to the Nimaviridae family, characterized by high virulence, rapid transmission, and a high mortality rate (80%).

EMS, or Acute Hepatopancreatic Necrosis Disease (AHPND), is induced by Vibrio parahaemolyticus, which secretes toxins that impair the hepatopancreas, resulting in high mortality rates within shrimp populations.

TopSPEC® WSSV/EMS PCR KIT is designed to detect the presence of both WSSV and EMS in shrimp samples using PCR technology. The kit offers quick testing time, high accuracy, a simple procedure, and the ability to detect multiple pathogens in a single amplification reaction.

TopSPEC® WSSV/EMS PCR KIT

Specification

The recommended extraction kit to use is: TopPURE ® Highpure Viral extraction kit (EXS-021)

The recommended electrophoresis kit to use is: TopSPEC® Agarose Electrophoresis (AEP-01)

Result analysis

– After electrophoresis is completed, the results are visualized on a UV transilluminator or a gel documentation system equipped with UV light.

– 100bp DNA ladder used includes 10 lines from 100 to 1000 bp

– Expected results are as follows:

• A positive sample with WSSV (White Spot Syndrome Virus) shows a band with a size of 526 bp (between the 500 bp and 600 bp markers).
• A positive sample with EMS (Early Mortality Syndrome) shows a band with a size of 316 bp (between the 200 bp and 300 bp markers).
• Internal control: appears band with size 102bp

Read more: Instructional Video for Qualitative Real-time PCR Kit