{"id":9725,"date":"2024-01-26T09:08:35","date_gmt":"2024-01-26T01:38:35","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=9725"},"modified":"2025-10-09T14:19:12","modified_gmt":"2025-10-09T06:49:12","slug":"topspec-v-vulnificus-v-alginolyticus-qpcr-kit-mqs-382","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-v-vulnificus-v-alginolyticus-qpcr-kit-mqs-382\/","title":{"rendered":"TopSPEC\u00ae V.vulnificus\/ V.alginolyticus qPCR Kit (MQS-382)"},"content":{"rendered":"<h4><strong><strong>Introduction<\/strong>\u00a0<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\"><em>Vibrio vulnificus<\/em> and <em>Vibrio alginolyticus<\/em> are Gram-negative bacteria belonging to the Vibrionaceae family, characterized by their motility, facultative anaerobic nature, and typically straight or slightly curved rod-shaped morphology. <em>V. vulnificus<\/em> is a known pathogen responsible for red body syndrome in postlarval shrimp and mass mortality in postlarval stages of Pacific white shrimp. On the other hand, <em>V. alginolyticus<\/em> is commonly recognized as the causative agent of red body syndrome in postlarval stages of Pacific white shrimp. Therefore, early detection of the presence of these bacteria in aquaculture environments is crucial to minimize the severe consequences they may cause.<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V.vulnificus\/ V.alginolyticus<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> <strong>qPCR Kit<\/strong> <\/span><\/strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">is a testing kit designed to detect the presence of <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V.vulnificus\/ V.alginolyticu<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>s<\/em> in aquatic samples using the Real-time PCR technique. The kit relies on the detection of vvhA gene and toxR gene, enabling the early and rapid identification of the pathogenic agents with high sensitivity and specificity.<\/span><\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V.vulnificus\/ V.alginolyticus<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> <strong>qPCR Kit<\/strong><\/span><\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong> <span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <em>V.vulnificus\/ V.alginolyticus<\/em><\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">\u00a0<strong>qPCR Kit<\/strong><\/span><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<table style=\"height: 499px;\" width=\"1121\">\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"414\"><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. vulnificus and V. alginolyticus \u2013 vvhA gene, toxR gene<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"414\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"414\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"414\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">FAM: <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. vulnificus<\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">Texas Red: <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. alginolyticus<\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"414\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"414\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"414\">Kit only detects <em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. vulnificus and V. alginolyticus<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"414\"><span class=\"WdYUQQ\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>V.vulnificus\/V.alginolyticus <\/em><\/span>qPCR mix, negative control, positive control, internal control, PCR tube<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"414\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit\u00a0<\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong><span class=\"WdYUQQ\"> Detect<span class=\"OYPEnA text-decoration-none text-strikethrough-none\">the presence of <em>V.vulnificus\/ V.alginolyticus<\/em><\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">\u00a0in aquatic samples using the Real-time PCR technique.<\/span><\/span><\/p>\n<p><strong>Storage<\/strong>: -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a vi khu\u1ea9n V.vulnificus\/V.alginolyticus\u00a0tr\u00ean m\u1eabu thu\u1ef7 s\u1ea3n b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR . \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9725","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=9725"}],"version-history":[{"count":7,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9725\/revisions"}],"predecessor-version":[{"id":13582,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9725\/revisions\/13582"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=9725"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=9725"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=9725"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=9725"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}