{"id":9700,"date":"2024-01-24T09:49:03","date_gmt":"2024-01-24T02:19:03","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=9700"},"modified":"2025-10-09T14:26:00","modified_gmt":"2025-10-09T06:56:00","slug":"topspec-v-parahaemolyticus-v-cholera-qpcr-kit","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-v-parahaemolyticus-v-cholera-qpcr-kit\/","title":{"rendered":"TopSPEC\u00ae V. parahaemolyticus\/ V. cholera qPCR KIT (MQS-392)"},"content":{"rendered":"<h4><strong>Introduction of TopSPEC\u00ae <em>V. parahaemolyticus\/ V. cholera<\/em> qPCR KIT<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\"><em>Vibrio parahaemolyticus<\/em> and <em>Vibrio cholera<\/em> are Gram-negative bacteria belonging to the Vibrionaceae family, characterized by their motility, facultative anaerobic nature, and typically straight or slightly curved rod-shaped morphology. <em>Vibrio parahaemolyticus<\/em> is notably recognized as a primary causative agent of Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Disease (AHPND), resulting in mass mortality and severe losses in shrimp aquaculture. Meanwhile, <\/span><a href=\"https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cholera?gclid=CjwKCAiA5L2tBhBTEiwAdSxJX4XddaLx0GvizLNZW84mA5s9RpzXNDGmeZ6WHGUisdutSc3ieVgZJBoCEh4QAvD_BwE\" target=\"_blank\" rel=\"noopener\"><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">Vibrio cholera<\/span><\/em><\/a><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> is commonly associated with acute intestinal inflammation in humans following the consumption of contaminated food (seafood, water, etc.).<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>V. parahaemolyticus<\/em><\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">\/<\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. cholera<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> <strong>qPCR Kit<\/strong> <\/span><\/strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">is a testing kit designed to detect the presence of <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. parahaemolyticus\/V. cholera<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> bacteria in aquatic samples using the Real-time PCR technique, based on the presence of the tlh gene and hlyA gene. This kit facilitates early detection of these pathogens, contributing to the mitigation of adverse effects caused by these bacteria.<\/span><\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>V. parahaemolyticus<\/em><\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">\/<\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. cholera<\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> <strong>qPCR Kit<\/strong><\/span><\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\">Targets<\/td>\n<td width=\"425\"><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. parahaemolyticus v\u00e0 V. cholera &#8211; tlh gene, hlyA gene<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Input sample<\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Input sample volume<\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Quantitative detection<\/td>\n<td width=\"425\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">FAM: <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. cholera<\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">Texas Red: <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>V. parahaemolyticus<\/em><\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Flourescent channel<\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">PCR time<\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Specificity<\/td>\n<td width=\"425\">Kit only detects <em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><em>V. parahaemolyticus<\/em><\/span><\/em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\"> and <\/span><em><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V. cholera<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Storage<\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\">Kit components<\/td>\n<td width=\"425\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">V.para\/V.cholera<\/span> qPCR mix, negative control, positive control, internal control, PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit <\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses<\/strong>: Detecting the presence of <em>V. parahaemolyticus\/V. cholera<\/em> bacteria in aquatic samples using<br \/>\nthe Real-time PCR technique, based on the presence of the tlh gene and hlyA gene.<\/p>\n<p><strong>Storage:<\/strong> -20\u00b0C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a vi khu\u1ea9n V. parahaemolyticus\/V. cholera tr\u00ean m\u1eabu thu\u1ef7 s\u1ea3n b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR d\u1ef1a tr\u00ean s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a tlh gene, hlyA gene. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20\u00b0C Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9700","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=9700"}],"version-history":[{"count":5,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9700\/revisions"}],"predecessor-version":[{"id":13584,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9700\/revisions\/13584"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=9700"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=9700"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=9700"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=9700"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}