{"id":9665,"date":"2024-01-22T10:45:34","date_gmt":"2024-01-22T03:15:34","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=9665"},"modified":"2025-10-07T17:04:11","modified_gmt":"2025-10-07T09:34:11","slug":"topspec-virotype-prrsv-rt-qpcr-kit-mqv-124","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-virotype-prrsv-rt-qpcr-kit-mqv-124\/","title":{"rendered":"TopSPEC\u00ae Virotype PRRSV RT-qPCR KIT (MQV-124)"},"content":{"rendered":"<h3><strong>Introduction of PRRSV<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\">The syndrome of respiratory and reproductive disorders in pigs, commonly known as <em>Porcine Reproductive and Respiratory Syndrome<\/em> (PRRS), is caused by the PRRSV. This is a highly contagious and dangerous infectious disease that spreads rapidly and leads to a significant mortality rate in infected pigs, resulting in substantial losses to the swine farming industry. The disease affects pigs of all ages, with a potential mortality rate of up to 100%. Early detection of the disease within a herd plays a crucial role in mitigating the damages caused by the illness.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-9666\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/01\/the-virus-main-img-300x291.png\" alt=\"PRRSV\" width=\"316\" height=\"307\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/01\/the-virus-main-img-300x291.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/01\/the-virus-main-img-768x744.png 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/01\/the-virus-main-img-12x12.png 12w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/01\/the-virus-main-img.png 913w\" sizes=\"(max-width: 316px) 100vw, 316px\" \/><\/p>\n<p style=\"text-align: center;\">Illustration of PRRSV virus that causes disease in pigs<\/p>\n<p><strong>TopSPEC\u00ae Virotype PRRSV RT-qPCR KIT<\/strong> <span class=\"OYPEnA text-decoration-none text-strikethrough-none\">is a diagnostic kit designed for the detection of PRRSV, which causes respiratory and reproductive disorders  in pigs. Additionally, the kit facilitates the differentiation of various PRRSV strains, including PRRSV type I (EU), PRRSV type II (NA), and highly pathogenic PRRSV Type II HP (gene OFR1a). By using the RT-qPCR technique, this kit enables detection across diverse sample backgrounds with high sensitivity and specificity.<\/span><\/p>\n<h3><strong><strong>Highlights<\/strong><\/strong><\/h3>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimized PCR time was approximately 2 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h3><strong><strong>Specification<\/strong><\/strong><\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">PRRSV type I (EU), PRRSV type II (NA) v\u00e0 PRRSV type II HP<\/span> &#8211; gen ORF1a<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">RNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: PRRSV type I (EU)<\/p>\n<p>Texas Red: PRRSV type II HP<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">Cy5: PRRSV type II (NA)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects <span class=\"OYPEnA text-decoration-none text-strikethrough-none\">PRRSV type I (EU), PRRSV type II (NA) v\u00e0 PRRSV type II HP<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">PRRSV One-step RT-qPCR mix, negative control, positive control, internal control (IC), PCR tube<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<p>Silica column extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid Viral Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-dna-rna-co-extraction-kit-hi-004\/\">TopPURE\u00ae Serum Viral DNA\/RNA Co-Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-viral-dna-rna-extraction-kit-he-008\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Viral DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<p>Automatic magnetic beads extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-quick-viral-extration-kit-hi-812-2\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Viral DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> <span class=\"OYPEnA text-decoration-none text-strikethrough-none\">Detection of the presence of PRRSV virus causing respiratory and reproductive syndrome in pigs and distinguish between PRRSV strains such as PRRSV type I (EU), PRRSV type II (NA) and PRRSV Type II highly virulent HP (OFR1a gene).<\/span><\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n virus PRRSV g\u00e2y h\u1ed9i ch\u1ee9ng r\u1ed1i lo\u1ea1n h\u00f4 h\u1ea5p v\u00e0 sinh s\u1ea3n \u1edf l\u1ee3n v\u00e0 ph\u00e2n bi\u1ec7t c\u00e1c ch\u1ee7ng PRRSV nh\u01b0 PRRSV type I (EU), PRRSV type II (NA) v\u00e0 PRRSV Type II \u0111\u1ed9c l\u1ef1c cao HP (gene OFR1a). \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9665","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=9665"}],"version-history":[{"count":7,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9665\/revisions"}],"predecessor-version":[{"id":13525,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/9665\/revisions\/13525"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=9665"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=9665"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=9665"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=9665"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}