{"id":9303,"date":"2023-12-05T09:47:40","date_gmt":"2023-12-05T02:17:40","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&p=9303"},"modified":"2025-10-09T09:25:59","modified_gmt":"2025-10-09T01:55:59","slug":"topspec-wssv-ems-ehp-vibrio-parahaemolyticus-pcr-kit","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-wssv-ems-ehp-vibrio-parahaemolyticus-pcr-kit\/","title":{"rendered":"TopSPEC\u00ae WSSV-EMS-EHP-Vibrio parahaemolyticus PCR KIT (SPS-306)"},"content":{"rendered":"

INTRODUCTION<\/h4>\n

 <\/p>\n

\u2013 White spot disease<\/em> is caused by the virus that causes white spot syndrome (White Spot Syndrome Virus \u2013 WSSV). This is a double-stranded DNA virus belonging to the Nimaviridae family, with strong virulence, rapid spread and high mortality rate (80%).<\/p>\n

- White spot disease<\/em> caused by the White Spot Syndrome Virus (WSSV). This is a double-stranded DNA virus belonging to the Nimaviridae family, characterized by high virulence, rapid transmission, and a high mortality rate (80%).<\/p>\n

\u2013 Microsporidiosis<\/em> caused by a parasite Enterocytozoon Hepatopenaei<\/em> (EHP). Shrimp infected with microsporidiosis usually exhibit slow growth because the parasite resides in the hepatopancreas and absorbs the shrimp\u2019s nutrients. Although the disease does not cause mass mortality, it leads to significant economic losses due to reduced yield.<\/p>\n

\u2013 Early Mortality Syndrome \u2013 EMS, also known as Acute Hepatopancreatic Necrosis Disease \u2013 AHPND, is caused by the bacteria Vibrio parahaemolyticus<\/i> . They parasitize in the intestinal tract of shrimp and secrete toxins that cause the liver to swell or shrink, causing mass deaths in ponds.<\/p>\n

TopSPEC\u00ae WSSV PCR KIT (TopSPEC\u00ae WSSV-EMS-EHP-Vibrio parahaemolyticus<\/em> PCR KIT)<\/strong> is a diagnostic kit designed to detect the presence of WSSV\/EMS\/EHP and V.parahaemolyticus<\/em> in shrimp samples using PCR technology.\nThe kit offers outstanding advantages such as rapid testing time, high accuracy, a simple workflow, and the ability to detect multiple pathogens in a single reaction.<\/p>\n

\"\"<\/h4>\n

TopSPEC\u00ae WSSV-EMS-EHP-Vibrio parahaemolyticus<\/em> PCR KIT<\/strong><\/p>\n

Specification<\/strong><\/strong><\/h4>\n\n\n\n\n\n\n\n\n\n\n
Targets<\/strong><\/td>\nWSSV – EMS – EHP – Vibrio parahaemolyticus <\/em><\/td>\n<\/tr>\n
Input sample<\/strong><\/td>\nShrimp tissue samples (gills, swimming feet, feces)<\/td>\n<\/tr>\n
Input sample volume<\/strong><\/td>\n200 \u00b5L<\/td>\n<\/tr>\n
DNA volume<\/strong><\/td>\n5 \u00b5L<\/td>\n<\/tr>\n
PCR time<\/strong><\/td>\n2 hours \u2013 2 hours 30 minutes (depending on the model)<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nKit only detects WSSV \u2013 EMS \u2013 EHP \u2013 Vibrio parahaemolyticus<\/em><\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n12 months at -20o<\/sup>C<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\nWEEV PCR Mix, negative control, positive control, tube PCR<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

The recommended extraction kit to use is: <\/strong>TopPURE \u00ae Highpure Viral extraction kit (EXS-021)<\/a><\/p>\n

The recommended electrophoresis kit to use is: <\/strong>TopSPEC\u00ae Agarose Electrophoresis (AEP-01)<\/a><\/p>\n

Result analysis<\/strong><\/p>\n

\u2013 After electrophoresis is completed, the results are visualized on a UV transilluminator or a gel documentation system equipped with UV light.<\/p>\n

\u2013 100bp DNA ladder used includes 10 lines from 100 to 1000 bp<\/p>\n

\u2013 Expected results are as follows:<\/p>\n