{"id":8691,"date":"2023-07-31T15:06:19","date_gmt":"2023-07-31T07:36:19","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=8691"},"modified":"2025-10-21T11:46:16","modified_gmt":"2025-10-21T04:16:16","slug":"toppure-hpv-pap-smear-collection-hi-016","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/toppure-hpv-pap-smear-collection-hi-016\/","title":{"rendered":"TopPURE\u00ae HPV Pap Smear Collection Kit (HI-016)"},"content":{"rendered":"<h2><strong>1. Information about Cervical Cancer and<\/strong><strong> HPV Pap Smear Kit<\/strong><\/h2>\n<p><strong>Cervical cancer<\/strong> is a disease associated with abnormal proliferation of cells in the cervical epithelium (the organ between the uterus and the vagina), forming tumors in the uterus. Abnormal cervical cells exhibit characteristics such as multinucleation, increased pigmentation, and non-keratinization around the nucleus. The nucleus of abnormal cells is three times larger than that of normal cells. Changes in nuclear shape and cellular substance form the basis for cytological methods in UTCTC early screening.<\/p>\n<p>Based on cell type, cervical cancer is divided into two main types: squamous cell carcinoma (SCC) and adenocarcinoma (AC). SCC occurs in the epithelial cells at the outer location of the cervix and is more common, while AC occurs in the endocrine glands of the cervix.<\/p>\n<p>The main cause of cervical cancer is Human papillomavirus (HPV), a virus that induces papillomas in humans and animals. <strong>HPV (<a href=\"https:\/\/www.who.int\/teams\/health-product-policy-and-standards\/standards-and-specifications\/vaccine-standardization\/human-papillomavirus\" target=\"_blank\" rel=\"noopener\">Human papillomavirus<\/a><\/strong>) is transmitted sexually through unprotected sexual activity. According to the World Health Organization (WHO), cervical cancer is the fourth most common cancer among women worldwide. Globocan statistics report that 185 out of 204 countries worldwide have data on cervical cancer. Among them, Vietnam ranks 91st out of 185 for incidence rate and 50th out of 185 for mortality rate globally.<\/p>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\">The <strong>TopPURE\u00ae HPV PAP SMEAR COLLECTION KIT<\/strong> is a set of tools for processing and collecting vaginal cell samples from smear samples. The collected vaginal smear sample, obtained from the cervical region, is processed to remove mucus and blood in the sample medium containing ethanol buffer. The preserved cells will maintain their shape (nucleus and cytoplasm) for a minimum period of 1 month under room temperature conditions (15\u201325\u00b0C). Additionally, the preserved cells can be used in qualitative HPV tests through HPV DNA analysis. The UTCTC screening sample collection kit (HI-016) includes:<\/p>\n<ol>\n<li><strong>The sampling stick NLD8303<\/strong> consists of: a handle, a sample collection tip, and a plunger. The sample collection tip is made of PE (polyethylene), while the handle and plunger are made of PP (polypropylene).<\/li>\n<li><strong>Pap smear solution<\/strong> is transparent, colorless, solution pH 5.5 \u00b1 0.2.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<h2><strong>2. Manual intructions\u00a0<\/strong><\/h2>\n<p><em><strong>\u2013 Sample collection procedure:<\/strong><\/em><\/p>\n<p>The collection of cervical smear samples will be carried out following the procedures outlined by the Ministry of Health.<\/p>\n<ul>\n<li>Step 1: Place the speculum in the vagina and adjust the position to ensure clear visibility of the cervix.<\/li>\n<li>Step 2: Insert the gynecological sampling brush into the cervical area, press gently, and rotate the brush clockwise 5 times (Figure 1).<\/li>\n<li>Step 3: Remove the brush from the vagina and gently rotate it in the Pap Smear Solution vial 5-10 times. Retain the brush head, collect the sample by detaching the head from the handle, and handle the brush appropriately.<\/li>\n<li>Step 4: Close the vial lid and record patient information on the label using a pencil.<\/li>\n<\/ul>\n<p>Store and transport the sample at room temperature (25\u00b0C) for up to 1 month for cytology testing (Pap test) in cervical cancer screening.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"size-medium wp-image-9497 aligncenter entered litespeed-loaded\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/12\/screenshot_1703494485-300x181.png\" alt=\"\" width=\"300\" height=\"181\" data-lazyloaded=\"1\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/12\/screenshot_1703494485-300x181.png\" data-ll-status=\"loaded\" \/><\/p>\n<p>\u2013\u00a0<em><strong>DNA extraction procedure \u2013 HPV:<\/strong><\/em><\/p>\n<p>The smears stored in Pap Smear Solution can be used for qualitative HPV Real-time PCR testing:<\/p>\n<ul>\n<li>Step 1: Aspirate 1 mL of the Pap Smear Solution-treated sample into a 1.5 mL tube. Centrifuge at 10,000 rpm for 3 minutes, collect the pellet, and discard the supernatant.<\/li>\n<li>Step 2: The pellet will be used for DNA extraction following the kit\u2019s protocol <em><strong><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit (HI-112)<\/a><\/strong><\/em>\u00a0- silica column extraction and\u00a0<a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\"><em><strong>TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit (HI-612)<\/strong><\/em><\/a>\u00a0- magnetic beads extraction.<\/li>\n<li>Step 3: Qualify the DNA after extraction using\u00a0<a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/topsensi-hpv-qpcr-kit\/\"><em><strong>TopSENSI\u00ae HPV qPCR Kit (SQH-103)<\/strong><\/em><\/a><\/li>\n<\/ul>\n<h2><strong>3. Precaution\u00a0<\/strong><\/h2>\n<ul>\n<li>Always wear gloves and a facemask during the procedure.<\/li>\n<li>Check the packaging and product carefully before use. If the product is damaged, do not use it.<\/li>\n<li>Each component in the kit is for single use only. Requirements of aseptic operations should be strictly followed. Discard immediately after use, do not reuse. Waste must be disposed in accordance with national environmental protection laws and\nregulations.<\/li>\n<li>The kit should be stored at a temperature of 15-25\u00b0C, relative humidity not more than 80%, good ventilation.<\/li>\n<li>The kit should avoid harsh condition such as high pressure, strong vibration, direct sunlight, high temperature, humidity, rain and corrosive gas or liquid during transportation.<\/li>\n<li>Carefully read the instructions for use, pay attention to the expiration date of the product before use. Do not use the product after the expiration date.<\/li>\n<\/ul>\n<p>Read more: <a href=\"https:\/\/abtvn.com\/en\/dinh-tuyp-hpv-bang-rdb\/\">HPV Genotype with RDB technique\u00a0<\/a><\/p>\n<p>For Real-time Kit: <a href=\"https:\/\/abtvn.com\/en\/product\/topsensi-hpv-6-11-16-18-hr-qpcr-kit-sqh-006\/\">TopSENSI \u00ae HPV 6, 11, 16, 18 &amp; High Risk qPCR Kit (SQH-006)<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses<\/strong>: Used for collecting and preserving gynecological cells for cytology analysis in order for cervical cancer screening (Pap smear). The sample can also be used to detection of HPV-DNA.<\/p>\n<p><strong>Storage: <\/strong>\u00a0Room temperature<\/p>\n<p><strong>Expiration Date<\/strong>: <strong>\u00a0<\/strong>12 months from manufacture.<\/p>\n<p><strong>Contraindication:<\/strong><\/p>\n<p>- Pregnant women.<\/p>\n<p>- Women currently menstruating.<\/p>\n<p>\u2013 Within a 2-day window before and after menstruation.<\/p>","protected":false},"featured_media":12204,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: D\u00f9ng \u0111\u1ec3 thu v\u00e0 b\u1ea3o qu\u1ea3n t\u1ebf b\u00e0o ph\u1ee5 khoa cho m\u1ee5c \u0111\u00edch ph\u00e2n t\u00edch t\u1ebf b\u00e0o h\u1ecdc t\u1ea7m so\u00e1t UTCTC (ph\u01b0\u01a1ng ph\u00e1p pap smear), ngo\u00e0i ra m\u1eabu c\u0169ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c x\u1eed l\u00fd \u0111\u1ec3 s\u1eed d\u1ee5ng cho m\u1ee5c \u0111\u00edch ph\u00e1t hi\u1ec7n HPV-DNA \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: \u00a0Nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng. H\u1ea1n s\u1eed d\u1ee5ng:&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8691","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=8691"}],"version-history":[{"count":7,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8691\/revisions"}],"predecessor-version":[{"id":13696,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8691\/revisions\/13696"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/12204"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=8691"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=8691"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=8691"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=8691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}