{"id":8453,"date":"2023-07-19T15:30:18","date_gmt":"2023-07-19T08:00:18","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=8453"},"modified":"2025-08-02T12:10:53","modified_gmt":"2025-08-02T04:40:53","slug":"6x-gelgreen-loading-buffer","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/6x-gelgreen-loading-buffer\/","title":{"rendered":"6X GELGREEN LOADING BUFFER WITH TRICOLOR"},"content":{"rendered":"<h3><strong>Specifications<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\"><strong>6X GelGreen Loading Buffer With Tricolor<\/strong> is a sensitive, stable, and environmentally safe green fluorescent nucleic acid dye, specially designed for gel staining. GelGreen offers significantly higher sensitivity compared to SYBR Safe. Unlike SYBR dyes\u2014which are known for their instability\u2014GelGreen is highly stable against both hydrolysis and thermal degradation.\nThis buffer is optimally excited at 497\u202fnm and also exhibits a secondary excitation peak at 248\u202fnm. Upon binding to DNA, its fluorescence emission is centered at 524\u202fnm. These spectral properties make <strong>6X GelGreen Loading Buffer With Tricolor<\/strong> compatible with a wide range of gel imaging systems.<\/p>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"size-medium wp-image-8456 aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-300x168.jpg\" alt=\"6X GelGreen Loading buffer With Tricolor\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-300x168.jpg 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-1024x575.jpg 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-768x431.jpg 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-1536x862.jpg 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-2048x1150.jpg 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-18x10.jpg 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen-1707x958.jpg 1707w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/6x-gelgreen.jpg 1920w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>6X GelGreen Loading buffer With Tricolor<\/strong><\/p>\n<h3><strong>Manual instruction<\/strong><\/h3>\n<ul>\n<li>Use distilled water to dilute 50X TAE buffer (or 10X TBE buffer) to 1X TAE (or 1X TBE) before use.<\/li>\n<li>Weigh the appropriate amount of agarose (depending on desired gel percentage) and place it in a suitable container.<\/li>\n<li>Add the correct volume of 1X TAE (or 1X TBE) buffer and mix well.<\/li>\n<li>Boil the mixture in the microwave until the agarose is completely dissolved.<\/li>\n<li>Cool the gel to 50-55 \u030aC, pour the gel into the mold and insert the comb. Be careful not to let air bubbles appear. If air bubbles appear, carefully push them to the sides with the tip of a pipette.<\/li>\n<li>Place the newly poured gel at a temperature of 4 \u030aC for about 10-15 minutes (in case of urgent need) or leave it at room temperature for about 20-30 minutes, until the gel is completely solidified.<\/li>\n<li>Once cooled, place the gel tray into the electrophoresis chamber. Remove the comb and add 1X TAE (or 1X TBE) buffer to the gel tank until the buffer level is about 2\u202fmm above the gel surface.<\/li>\n<li class=\"translation-block\">PCR products loading: Mix 5\u202f\u03bcL of PCR product with 1\u202f\u03bcL of <strong>6X GelGreen Loading Buffer With Tricolor<\/strong> and load into each well. DNA ladder: Mix 5\u202f\u03bcL of DNA ladder with 1\u202f\u03bcL of <strong>6X GelGreen Loading Buffer With Tricolor<\/strong>.<\/li>\n<li>Electrophoresis: Run the gel at 85\u202fV for 30 minutes, or adjust voltage and time according to experimental needs.<\/li>\n<\/ul>\n<h3><strong>Caution<\/strong><\/h3>\n<ul>\n<li>Use appropriate voltage and duration for each electrophoresis chamber.<\/li>\n<li>Ensure correct electrode placement during electrophoresis.<\/li>\n<li>Regularly clean and UV-sterilize the electrophoresis room.<\/li>\n<li>Avoid direct contact via inhalation, skin, eyes, or ingestion.<\/li>\n<\/ul>\n<p>Read more:<\/p>\n<ul>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product-category\/hoa-chat\/hoa-chat-shpt-co-ban\/\">Other Bio-reagents<\/a><\/li>\n<li><a class=\"connectButton\" href=\"https:\/\/drive.google.com\/drive\/u\/0\/folders\/1F8WgOeV9-4E1VNR4i5uwL-kOevp4eK-x\" target=\"_blank\" rel=\"noopener\">Manual instruction<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose: 6x Gelgreen Loading Buffer <\/strong> is a green fluorescent nucleic acid dye designed for staining in agarose gels. It serves as a safer alternative to Ethidium Bromide (EtBr), a highly toxic compound.<\/p>\n<p><strong>Storage:<\/strong> 2-8\u00b0C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p class=\"translation-block\"><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":8456,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch:\u00a0 6x Gelgreen Loading Buffer l\u00e0 m\u1ed9t lo\u1ea1i thu\u1ed1c nhu\u1ed9m acid nucleic hu\u1ef3nh quang m\u00e0u xanh l\u00e1 c\u00e2y \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 nhu\u1ed9m trong gel agarose thay th\u1ebf Ethidium bromide (EtBr) \u2013 ch\u1ea5t c\u00f3 t\u00ednh \u0111\u1ed9c h\u1ea1i cao. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: 2-8\u00b0C H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng:&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=8453"}],"version-history":[{"count":6,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8453\/revisions"}],"predecessor-version":[{"id":13014,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8453\/revisions\/13014"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/8456"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=8453"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=8453"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=8453"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=8453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}