{"id":8451,"date":"2023-07-19T15:18:24","date_gmt":"2023-07-19T07:48:24","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=8451"},"modified":"2025-08-26T17:30:48","modified_gmt":"2025-08-26T10:00:48","slug":"gelred-3x-in-water","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/gelred-3x-in-water\/","title":{"rendered":"GELRED 3X IN WATER"},"content":{"rendered":"<h2><strong>Specifications<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p><strong>GelRed 3X in water<\/strong> is an ultrasensitive, highly stable, and environmentally safe fluorescent nucleic acid staining solution designed to replace ethidium bromide (EtBr), a highly toxic compound. <strong>GelRed 3X in water<\/strong> is used for staining double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), or RNA in agarose or polyacrylamide gels. GelRed and EtBr exhibit nearly identical spectral properties, allowing GelRed to be directly substituted for EtBr without requiring modifications to existing imaging systems.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"size-medium wp-image-8458 aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-300x168.jpg\" alt=\"gelred-3x-in-wate\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-300x168.jpg 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-1024x575.jpg 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-768x431.jpg 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-1536x862.jpg 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-2048x1149.jpg 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-18x10.jpg 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-1707x958.jpg 1707w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/07\/gelred-3x-in-water-scaled.jpg 1920w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>GelRed 3X in water \u2013 100mL Bottle<\/strong><\/p>\n<h2><strong>Manual instruction<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"text-decoration: underline;\">Method 1:<\/span> <em>Post-Electrophoresis Gel Staining with GelRed 3X in Water<\/em><\/strong><\/p>\n<ul>\n<li>Step 1: Cast the gel by pouring molten agarose into a mold with a comb in place. Allow the gel to solidify at room temperature.<\/li>\n<li>Step 2: Load samples and perform electrophoresis according to standard procedures.<\/li>\n<li>Step 3: Prepare the staining solution by diluting <strong>GelRed 3X in water<\/strong> to a final concentration of 1X using H<sub>2<\/sub>O (Example: Mix 20 mL of <strong>GelRed 3X in water<\/strong> with 40 mL of H<sub>2<\/sub>O to obtain 60 mL of GelRed 1X).<\/li>\n<li>Step 4: Submerge the gel in the GelRed 1X solution, ensuring the liquid covers the gel by at least 2 mm. Incubate for 10\u201315 minutes in the dark.<\/li>\n<li>Step 5: Visualize the stained gel using a standard UV transilluminator (302 nm or 312 nm) and capture images using a UV light filter.<\/li>\n<\/ul>\n<p><strong><span style=\"text-decoration: underline;\">Method 2:<\/span> <em>Pre-Casting Gel with GelRed 3X in Water<\/em><\/strong><\/p>\n<ul>\n<li>Step 1: Dilute <strong>GelRed 3X in water<\/strong> to 1X using concentrated electrophoresis buffer.\nExample: Mix 20 mL of <strong>GelRed 3X in water<\/strong> with 6 mL of 10X TBE buffer and 34 mL of H<sub>2<\/sub>O to obtain 60 mL of 1X GelRed in 1X TBE).<\/li>\n<li>Step 2: Add the required amount of agarose to the GelRed 1X solution, mix thoroughly, and heat until fully dissolved.<\/li>\n<li>Step 3: Pour the molten gel into a mold with a comb in place and allow it to solidify at room temperature.<\/li>\n<\/ul>\n<p><span style=\"text-decoration: underline;\"><em>Note:<\/em><\/span><\/p>\n<p>- Unused gel solution can be stored and reheated for future use.<\/p>\n<p>- Molten gel (at ~50\u00b0C) should be used within 1\u20132 days.<\/p>\n<p>- Cast gels can be stored at room temperature in the dark for up to one week.<\/p>\n<p>- Avoid storing cast gels at 4\u00b0C, as this may cause dye precipitation and reduce staining efficiency.<\/p>\n<ul>\n<li>Step 4: Load samples and perform electrophoresis as usual.<\/li>\n<li>Step 5: Visualize the stained gel using a standard UV transilluminator (302 nm or 312 nm) and capture images using a UV light filter.<\/li>\n<\/ul>\n<h2><strong>Recommendations<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p>- Design protocols tailored to sample type and intended application.<\/p>\n<p>- Use appropriate voltage and run time for each electrophoresis chamber.<\/p>\n<p>- Ensure correct electrode placement during electrophoresis.<\/p>\n<p>- Regularly clean and UV-sterilize the electrophoresis area. Consider using <a href=\"https:\/\/abtvn.com\/en\/product\/topclean-dna-rna-away\/\">TopCLEAN \u00ae DNA\/RNA AWAY<\/a> to eliminate residual DNA\/RNA and prevent cross-contamination.<br \/>\n- Avoid direct exposure to GelRed via inhalation, skin contact, eye contact, or ingestion.<\/p>\n<p>Read more:<\/p>\n<ul>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product-category\/hoa-chat\/hoa-chat-shpt-co-ban\/\">Other Bio-reagents<\/a><\/li>\n<li><a class=\"connectButton\" href=\"https:\/\/drive.google.com\/drive\/u\/0\/folders\/1f7br7_Aab5DDsTh_3SSWMZ9NHDNYIhgD\" target=\"_blank\" rel=\"noopener\">Manual instruction<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> <strong>GelRed 3X in water<\/strong> is a fluorescent nucleic acid stain used for the detection of nucleic acids in agarose gel or polyacrylamide gel electrophoresis.<\/p>\n<p><strong>Storage:<\/strong> Cool temperature, 2\u20138\u202f\u00b0C.<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Package:\u00a0<\/strong>100ml Bottle (DD-013.100) v\u00e0 500ml Bottle (DD-013.500)<\/p>\n<p><strong>Manufacturer:<\/strong> ABT Vietnam<\/p>","protected":false},"featured_media":8458,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: GelRed 3X in water l\u00e0 ch\u1ea5t nhu\u1ed9m nucleic acid ph\u00e1t hu\u1ef3nh quang, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t hi\u1ec7n nucleic acid trong \u0111i\u1ec7n di gel agarose ho\u1eb7c gel polyacrylamide. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: Nhi\u1ec7t \u0111\u1ed9 m\u00e1t 2-8\u00b0C H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t Quy c\u00e1ch \u0111\u00f3ng g\u00f3i:\u00a0Chai 100ml (DD-013.100) v\u00e0&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8451","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=8451"}],"version-history":[{"count":6,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8451\/revisions"}],"predecessor-version":[{"id":13116,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8451\/revisions\/13116"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/8458"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=8451"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=8451"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=8451"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=8451"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}