{"id":8426,"date":"2023-07-19T11:59:50","date_gmt":"2023-07-19T04:29:50","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=8426"},"modified":"2025-08-26T17:30:48","modified_gmt":"2025-08-26T10:00:48","slug":"tbe-buffer-10x","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/tbe-buffer-10x\/","title":{"rendered":"TBE BUFFER 10X (Tris-borate-EDTA)"},"content":{"rendered":"<p><strong>Specifications<\/strong><\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\">TBE Buffer 10X (Tris-Borate-EDTA) l\u00e0 b\u1ed9 \u0111\u1ec7m s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn cho \u0111i\u1ec7n di DNA, RNA tr\u00ean gel polyacrylamide. TBE c\u0169ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho gel agarose, nh\u01b0ng kh\u00f4ng th\u00edch \u0111\u1ec3 thu h\u1ed3i axit nucleic. \u0110\u01b0\u1ee3c s\u1eed d\u1ee5ng nh\u01b0 \u0111\u1ec7m \u0111\u1ec3 ch\u1ea1y \u0111i\u1ec7n di v\u00e0 chu\u1ea9n b\u1ecb gel. N\u00ean s\u1eed d\u1ee5ng cho \u0111i\u1ec7n di c\u00e1c \u0111o\u1ea1n RNA v\u00e0 DNA nh\u1ecf h\u01a1n 1500 bp. S\u1ea3n ph\u1ea9m \u1edf d\u1ea1ng dung d\u1ecbch \u0111\u1ed3ng nh\u1ea5t, trong su\u1ed1t. B\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng.<\/p>\n<p><strong>Manual instruction<\/strong><\/p>\n<ul>\n<li>S\u1eed d\u1ee5ng n\u01b0\u1edbc c\u1ea5t \u0111\u1ec3 pha dung d\u1ecbch \u0111\u1ec7m TBE 10X th\u00e0nh TBE 1X tr\u01b0\u1edbc khi s\u1eed d\u1ee5ng.<\/li>\n<li>Weigh the appropriate amount of agarose (depending on the gel percentage) into a bottle of appropriate volume.<\/li>\n<li>Th\u00eam l\u01b0\u1ee3ng \u0111\u1ec7m TBE 1X ph\u00f9 h\u1ee3p v\u00e0 l\u1eafc \u0111\u1ec1u.<\/li>\n<li>Boil the mixture in the microwave until the agarose is completely dissolved.<\/li>\n<li>Cool the gel to 50-55 \u030aC, pour the gel into the mold and insert the comb. Be careful not to let air bubbles appear. If air bubbles appear, carefully push them to the sides with the tip of a pipette.<\/li>\n<li>Place the newly poured gel at a temperature of 4 \u030aC for about 10-15 minutes (in case of urgent need) or leave it at room temperature for about 20-30 minutes, until the gel is completely solidified.<\/li>\n<li>Khi gel ngu\u1ed9i, \u0111\u1eb7t khu\u00f4n gel v\u00e0o bu\u1ed3ng \u0111i\u1ec7n di, th\u00e1o l\u01b0\u1ee3c v\u00e0 th\u00eam \u0111\u1ec7m TBE 1X v\u00e0o khay gel sao cho \u0111\u1ec7m cao h\u01a1n m\u1eb7t gel kho\u1ea3ng 2 mm.<\/li>\n<li class=\"translation-block\">Load samples proportionally: Load samples proportionally: 5\u03bcL PCR product + 1\u03bcL 6X <a href=\"https:\/\/abtvn.com\/en\/product\/6x-gelred-loading-buffer\/\" class=\"connectButton\" target=\"_self\">GelRed Loading Buffer<\/a> into each well. The DNA ladder uses a ratio of 5 \u03bcL of DNA ladder and 1\u03bcL <a href=\"https:\/\/abtvn.com\/en\/product\/6x-gelred-loading-buffer\/\" class=\"connectButton\" target=\"_self\"> 6X GelRed Loading Buffer<\/a><\/li>\n<li>\u0110i\u1ec7n di \u1edf hi\u1ec7u \u0111i\u1ec7n th\u1ebf 85 V trong 30 ph\u00fat ho\u1eb7c \u0111\u1ebfn khi v\u1ea1ch ch\u1ec9 th\u1ecb m\u00e0u v\u00e0ng \u0111\u1ebfn ph\u00eda cu\u1ed1i c\u1ee7a gel. 3. C\u1ea3nh b\u00e1o<\/li>\n<\/ul>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:\u00a0<\/strong>TBE buffer \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong \u0111i\u1ec7n di gel agarose v\u00e0 gel polyacrylamide.<\/p>\n<p><strong>Storage:<\/strong> Room temperature in a dry and well-ventilated place.<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p class=\"translation-block\"><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":8438,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch:\u00a0TBE buffer \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong \u0111i\u1ec7n di gel agarose v\u00e0 gel polyacrylamide. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: Nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng \u1edf n\u01a1i kh\u00f4 r\u00e1o, tho\u00e1ng m\u00e1t. H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vietnam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8426","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=8426"}],"version-history":[{"count":1,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8426\/revisions"}],"predecessor-version":[{"id":8439,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/8426\/revisions\/8439"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/8438"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=8426"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=8426"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=8426"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=8426"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}