{"id":7766,"date":"2023-03-24T15:12:23","date_gmt":"2023-03-24T07:42:23","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7766"},"modified":"2025-11-27T10:53:31","modified_gmt":"2025-11-27T03:23:31","slug":"topspec-emcv-rt-qpcr-kit-sqv-119","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-emcv-rt-qpcr-kit-sqv-119\/","title":{"rendered":"TopSPEC\u00ae EMCV RT-qPCR KIT (SQV-119)"},"content":{"rendered":"<h4><strong>Gi\u1edbi thi\u1ec7u TopSPEC\u00ae EMCV RT-qPCR KIT<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p><strong>Encephalomyocarditis virus (EMCV) <\/strong>is a single-stranded RNA virus (ssRNA) belonging to the Cardiovirus genus within the Picornaviridae family. It is known as the primary causative agent of encephalomyocarditis (EMC) in pigs. EMCV can lead to mortality rates of up to 100% in piglets and result in impaired reproductive function and miscarriages in sows. Piglets infected with EMCV often experience myocarditis, encephalitis, and sudden death due to cardiac failure. Therefore, controlling and diagnosing EMCV helps minimize severe economic consequences for the pig farming industry.<\/p>\n<p><strong>TopSPEC\u00ae EMCV RT-qPCR KIT<\/strong> is designed to detect the presence of EMCV, specifically targeting the 5\u2019NTR gene, which causes the disease in pigs, using RT-qPCR technology. This kit facilitates rapid EMCV detection with a simple procedure and high sensitivity and specificity.<\/p>\n<picture><img fetchpriority=\"high\" decoding=\"async\" class=\"lazyautosizes lazyloaded wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae EMCV One-step RT-qPCR KIT\" width=\"300\" height=\"168\" data-eio=\"p\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-1024x575.jpeg.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-768x431.jpeg.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-18x10.jpeg.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1.jpeg.webp 1536w\" data-sizes=\"auto\" data-eio-rwidth=\"300\" data-eio-rheight=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/picture>\n<p style=\"text-align: center;\"><strong>TopSPEC\u00ae EMCV RT-qPCR KIT<\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time is optimized for 2 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><em>Encephalomyocarditis virus (EMCV) &#8211; gene 5\u2019NTR<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">Heart or brain tissue sample; Environmental surface swab sample<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>RNA volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: EMCV<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects EMCV<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">EMCV RT-qPCR mix, ch\u1ee9ng \u00e2m, ch\u1ee9ng d\u01b0\u01a1ng, ch\u1ee9ng n\u1ed9i, tube PCR<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid viral extraction kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA extraction kit<\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>Detecting the presence of Encephalomyocarditis virus (EMCV) - specifically targeting the 5\u2019NTR gene, in pigs, utilizing Real-time PCR technology.<\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a virus EMCV - gene 5\u2019NTR g\u00e2y b\u1ec7nh tr\u00ean heo (l\u1ee3n) b\u1eb1ng k\u1ef9 thu\u1eadt RT-qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT Vietnam","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7766","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7766"}],"version-history":[{"count":13,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7766\/revisions"}],"predecessor-version":[{"id":13955,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7766\/revisions\/13955"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7766"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7766"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7766"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7766"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}