{"id":7645,"date":"2023-03-07T16:02:26","date_gmt":"2023-03-07T08:32:26","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7645"},"modified":"2025-10-07T16:43:24","modified_gmt":"2025-10-07T09:13:24","slug":"topspec-adv-multiplex-qpcr-kit-mqv-123","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-adv-multiplex-qpcr-kit-mqv-123\/","title":{"rendered":"TopSPEC\u00ae ADV Multiplex qPCR KIT (MQV-123)"},"content":{"rendered":"<h4><strong><strong>Introduction<\/strong> <span class=\"S1PPyQ\">TopSPEC\u00ae ADV Multiplex qPCR Kit<\/span><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body translation-block\">Aujeszky's disease (AD) is a highly infectious and dangerous disease that affects pigs of all ages, with a high mortality rate. The disease is caused by a DNA virus belonging to the Herpesviridae family (PRV). AD causes symptoms such as fever, cough, runny nose, pneumonia, and abortion, as well as fetal death in sows. Currently, the most effective measure to control and minimize the damage caused by AD is whole-herd vaccination.<\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\"><span class=\"S1PPyQ\"><strong>TopSPEC\u00ae ADV Multiplex qPCR Kit<\/strong> <\/span><span class=\"S1PPyQ\">is a diagnostic assay designed to detect and differentiate pigs infected with wild-type Aujeszky\u2019s Disease Virus (ADV\/PRV), which carries the Glycoprotein B gene, from those vaccinated with inactivated strains lacking the Glycoprotein E gene. Utilizing real-time PCR technology, the kit enables rapid detection of ADV with high sensitivity and specificity.<\/span><\/p>\n<picture><img fetchpriority=\"high\" decoding=\"async\" class=\"lazyautosizes lazyloaded wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae ADV Multiplex qPCR Kit\" width=\"300\" height=\"168\" data-eio=\"p\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-1024x575.jpeg.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-768x431.jpeg.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-18x10.jpeg.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1.jpeg.webp 1536w\" data-sizes=\"auto\" data-eio-rwidth=\"300\" data-eio-rheight=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/picture>\n<p style=\"text-align: center;\"><span class=\"S1PPyQ\"><strong>TopSPEC\u00ae ADV Multiplex qPCR Kit<\/strong><\/span><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time is optimized for 2 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\">Wild-type ADV (PRV) carrying the Glycoprotein B gene or inactivated vaccine strains lacking the Glycoprotein E gene.\"<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: <span class=\"S1PPyQ\">detects Glycoprotein B gene,<\/span><\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\"><span class=\"S1PPyQ\">Texas Red: detects Glycoprotein E gene,<\/span><\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\"><span class=\"S1PPyQ\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detect <span class=\"S1PPyQ\">wild type ADV and vaccine strain<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">ADV multiplex qPCR mix, negative control, positive control, internal control, PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table>\n<tbody>\n<tr>\n<td colspan=\"3\" width=\"458\"><strong>Recommended for extraction kit<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><strong> Silica column extraction<\/strong><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-blood-dna-extraction-kit-he-007\/\">TopPURE\u00ae Blood DNA Extraction Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-dna-rna-co-extraction-kit-hi-004\/\">TopPURE\u00ae Serum Viral DNA\/RNA Co-Extraction Kit<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>Detection and differentiation of pigs infected with wild-type Aujeszky\u2019s Disease Virus (ADV\/PRV) carrying the Glycoprotein B gene, versus those vaccinated with inactivated strains lacking the Glycoprotein E gene, using qPCR technology.<\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n v\u00e0 ph\u00e2n bi\u1ec7t heo (l\u1ee3n) b\u1ecb nhi\u1ec5m virus ADV (PRV) hoang d\u1ea1i - gene Glycoprotein B hay nhi\u1ec5m ch\u1ee7ng vaccine b\u1ea5t ho\u1ea1t \u0111\u00e3 x\u00f3a gene Glycoprotein E b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7645"}],"version-history":[{"count":16,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7645\/revisions"}],"predecessor-version":[{"id":13521,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7645\/revisions\/13521"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7645"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7645"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7645"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}