{"id":7643,"date":"2023-03-07T15:49:22","date_gmt":"2023-03-07T08:19:22","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7643"},"modified":"2025-10-08T11:15:34","modified_gmt":"2025-10-08T03:45:34","slug":"topspec-adv-qpcr-kit-sqv-114","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-adv-qpcr-kit-sqv-114\/","title":{"rendered":"TopSPEC\u00ae ADV qPCR KIT (SQV-114)"},"content":{"rendered":"<h4><strong><strong>Introduction<\/strong> <span class=\"S1PPyQ\">TopSPEC\u00ae ADV qPCR Kit<\/span><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\"><strong><span class=\"S1PPyQ\">Pseudorabies (PR)<\/span><\/strong> , also known as Aujeszky\u2019s Disease (AD), is a viral disease that affects pigs and other animals such as dogs, cats, and cattle. It is caused by the Pseudorabies virus (PRV), a member of the Herpesviridae family. ADV<span class=\"S1PPyQ\">\u00a0infection in pigs leads to clinical symptoms including fever, anorexia, respiratory distress, and neurological signs. Infected pigs may also exhibit behavioral changes such as aggression or depression. The virus can be transmitted among pigs through direct contact with infected animals, as well as indirectly via contaminated equipment, feed, and clothing. Although ADV is a zoonotic disease, transmission to humans is extremely rare.<\/span><\/p>\n<p class=\"_04xlpA direction-ltr align-justify para-style-body\"><strong><span class=\"S1PPyQ\">TopSPEC\u00ae ADV qPCR Kit<\/span><\/strong><span class=\"S1PPyQ\"> s a diagnostic tool designed for the detection of ADV (PRV) \u2013 specifically the Glycoprotein B gene \u2013 in pigs using Real-time PCR technology. Early detection of the pathogen is critical for preventing the severe consequences associated with ADV infection.<\/span><\/p>\n<picture><img fetchpriority=\"high\" decoding=\"async\" class=\"lazyautosizes lazyloaded wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae ADV qPCR Kit\" width=\"300\" height=\"168\" data-eio=\"p\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-1024x575.jpeg.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-768x431.jpeg.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-18x10.jpeg.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1.jpeg.webp 1536w\" data-sizes=\"auto\" data-eio-rwidth=\"300\" data-eio-rheight=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/picture>\n<p style=\"text-align: center;\"><strong><span class=\"S1PPyQ\">TopSPEC\u00ae ADV qPCR Kit<\/span><\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><span class=\"S1PPyQ\">Aujeszky\u2019s, Pseudorabies (ADV-PRV) \u2013 Glycoprotein B gene\u00a0<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: Glycoprotein B gene of ADV<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detect<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">ADV qPCR mix, negative control, positive control, internal control (IC), PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table>\n<tbody>\n<tr>\n<td colspan=\"3\" width=\"458\"><strong>Recommended for extraction kit<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><strong> Silica column extraction<\/strong><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-blood-dna-extraction-kit-he-007\/\">TopPURE\u00ae Blood DNA Extraction Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"89\"><\/td>\n<td width=\"293\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-dna-rna-co-extraction-kit-hi-004\/\">TopPURE\u00ae Serum Viral DNA\/RNA Co-Extraction Kit<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>Detection of Aujeszky\u2019s Disease Virus (ADV\/PRV) causing pseudorabies in pigs through the Glycoprotein B gene using qPCR technique.<\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a virus ADV (PRV) g\u00e2y b\u1ec7nh gi\u1ea3 d\u1ea1i tr\u00ean heo (l\u1ee3n) th\u00f4ng qua gene Glycoprotein B b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT Vietnam","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7643","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7643"}],"version-history":[{"count":10,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7643\/revisions"}],"predecessor-version":[{"id":13544,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7643\/revisions\/13544"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7643"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7643"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7643"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7643"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}