{"id":7564,"date":"2023-02-28T13:48:44","date_gmt":"2023-02-28T06:18:44","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7564"},"modified":"2025-10-09T14:08:54","modified_gmt":"2025-10-09T06:38:54","slug":"topspec-mrnv-xsv-rt-qpcr-kit-mqs-342","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-mrnv-xsv-rt-qpcr-kit-mqs-342\/","title":{"rendered":"TopSPEC\u00ae MrNV\/XSV One-step RT-qPCR Kit (MQS-342)"},"content":{"rendered":"<h2><strong>INTRODUCTION<\/strong><\/h2>\n<p class=\"translation-block\"><strong>White tail disease (WTD)<\/strong> or <strong>white muscle disease (WMD)<\/strong> occurs in freshwater prawns caused by <strong>Macrobrachium Rosenbergii Nodavirus (MrNV)<\/strong> and <strong>Extra Small Virus (XSV)<\/strong>. Although MrNV is the main cause of the disease, XSV plays an equally important role. XSV is the first nodavirus-related satellite recorded in animals, and is limited to gill tissue, heart, ovary, and head muscle. Therefore, the disease can spread from shrimp seeds to larvae and even the early stages of juveniles. The mortality rate reaches 100% within 5-6 days from the onset of the disease.<\/p>\n<p class=\"translation-block\"><strong>TopSPEC\u00ae MrNV\/XSV One-step RT-qPCR Kit<\/strong> is a test kit that detects the presence of Macrobrachium Rosenbergii Nodavirus (MrNV) and Extra Small Virus (XSV) in the culture environment. Early detection of pathogens helps save costs caused by MrNV\/XSV.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><em>The <strong>TopSPEC\u00ae MrNV\/XSV One-step RT-qPCR Kit <\/strong>(undivided master mix)<\/em><\/p>\n<h2><strong><strong>Highlights<\/strong><\/strong><\/h2>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time was optimized for 2 hours<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h2><strong><strong>Specification<\/strong><\/strong><\/h2>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\">Macrobrachium rosenbergii nodavirus (MrNV) v\u00e0 Extra Small Virus (XSV) &#8211; RNA dependent RNA Polymerase v\u00e0 capsid protein gene<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">RNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>RNA volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: MrNV, Cy5: XSV<br \/>\nHEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detect MrNV\/XSV<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">MX One-step RT- qPCR mix, negative control, positive control (MrNV, XSV), internal control, PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-highpure-viral-extraction-kit-exs-012\/\">TopPURE\u00ae Highpure viral extraction kit (EXS-021)<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit (HI-612)<\/a><\/p>\n<p><strong>Read more<\/strong>: <a href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o&amp;t=38s\" target=\"_blank\" rel=\"noopener\">Instructional video for the qualitative Real-time PCR kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose:<\/strong> Check for the simultaneous presence of MrNV\/XSV agents on shrimp samples using Real-time PCR technique.<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n \u0111\u1ed3ng th\u1eddi c\u1ee7a c\u00e1c t\u00e1c nh\u00e2n MrNV\/XSV tr\u00ean m\u1eabu t\u00f4m b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7564"}],"version-history":[{"count":13,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7564\/revisions"}],"predecessor-version":[{"id":13580,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7564\/revisions\/13580"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7564"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7564"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7564"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}