{"id":7558,"date":"2023-02-28T12:02:24","date_gmt":"2023-02-28T04:32:24","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7558"},"modified":"2025-10-09T10:27:37","modified_gmt":"2025-10-09T02:57:37","slug":"topspec-ahpnd-ems-qpcr-kit-sqs-152","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-ahpnd-ems-qpcr-kit-sqs-152\/","title":{"rendered":"TopSPEC\u00ae AHPND\/EMS qPCR Kit (SQS-152)"},"content":{"rendered":"<h4><strong>INTRODUCTION<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p>\u2018\u2019<em>Acute Hepatopancreatic Necrosis Disease<\/em>\u2019\u2019<strong> AHPND<\/strong> was initially referred to as \"<strong>EMS<\/strong>\u2019\u2019 <strong>(Early Mortality Syndrome)<\/strong> caused by the bacterium Vibrio parahaemolyticus. Once inside the shrimp, this bacterium produces potent toxins that destroy tissue and disrupt hepatopancreatic function. This is also the main cause of early mortality in shrimp within 30\u201335 days after stocking, with a mortality rate of up to 70%.<\/p>\n<p><strong>TopSPEC\u00ae AHPND\/EMS qPCR KIT<\/strong> is a diagnostic kit designed to detect the bacteria responsible for Acute Hepatopancreatic Necrosis Disease (AHPND) and Early Mortality Syndrome (EMS) in shrimp samples using Real-time PCR technology in aquaculture environments.\nThe kit offers several outstanding advantages, including a simple and rapid workflow, high sensitivity, and excellent specificity.<\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong>TopSPEC\u00ae AHPND\/EMS qPCR KIT<\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\">Acute Hepatopancreatic Necrosis Disease\/ Early Mortality Syndrome &#8211; PirA\/B gene<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">DNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: detection of AHPND\/EMS<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects AHPND\/EMS<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">EMS qPCR mix, negative control, positive control, internal control, PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-highpure-viral-extraction-kit-exs-012\/\">TopPURE\u00ae Highpure viral extraction kit (EXS-021)<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid Viral Extraction Kit (HI-712)<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit (HI-612)<\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose:<\/strong> Check and detect AHPND\/EMS syndrome on shrimp samples using Real-time PCR technique<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra ph\u00e1t hi\u1ec7n h\u1ed9i ch\u1ee9ng AHPND\/EMS tr\u00ean m\u1eabu t\u00f4m b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7558","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7558"}],"version-history":[{"count":13,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7558\/revisions"}],"predecessor-version":[{"id":13568,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7558\/revisions\/13568"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7558"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7558"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7558"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7558"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}