{"id":7532,"date":"2023-02-28T09:39:08","date_gmt":"2023-02-28T02:09:08","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7532"},"modified":"2025-11-27T10:30:12","modified_gmt":"2025-11-27T03:00:12","slug":"topspec-prrsv-rt-qpcr-kit","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-prrsv-rt-qpcr-kit\/","title":{"rendered":"TopSPEC\u00ae PRRSV RT-qPCR KIT (SQV-106)"},"content":{"rendered":"<h4><strong>Gi\u1edbi thi\u1ec7u TopSPEC\u00ae PRRSV RT-qPCR KIT<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\">The syndrome of respiratory and reproductive disorders in pigs, commonly known as <em>Porcine Reproductive and Respiratory Syndrome<\/em> (PRRS), is caused by the PRRSV. This is a highly contagious and dangerous infectious disease that spreads rapidly and leads to a significant mortality rate in infected pigs, resulting in substantial losses to the swine farming industry. The disease affects pigs of all ages, with a potential mortality rate of up to 100%. Early detection of the disease within a herd plays a crucial role in mitigating the damages caused by the illness.<\/p>\n<p><strong>TopSPEC\u00ae PRRSV RT-qPCR KIT<\/strong> is a diagnostic assay designed to detect the presence of PRRSV, the causative agent of blue ear disease - Porcine Reproductive and Respiratory Syndrome, in pigs. Additionally, the kit enables differentiation between PRRSV Type II (North American lineage), characterized by the ORF7 gene, and the highly pathogenic Type II (HP), characterized by the ORF1a gene, using One-step RT-qPCR.<\/p>\n<picture><img fetchpriority=\"high\" decoding=\"async\" class=\"lazyautosizes lazyloaded wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae PRRSV One-step RT-qPCR KIT\" width=\"300\" height=\"168\" data-eio=\"p\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-1024x575.jpeg.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-768x431.jpeg.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-18x10.jpeg.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1.jpeg.webp 1536w\" data-sizes=\"auto\" data-eio-rwidth=\"300\" data-eio-rheight=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/picture>\n<p style=\"text-align: center;\"><strong>TopSPEC\u00ae PRRSV RT-qPCR KIT<\/strong><\/p>\n<p><strong><strong>Highlights<\/strong><\/strong><\/p>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time is optimized for 2 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<p><strong><strong>Specification<\/strong><\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\">Porcine reproductive and respiratory syndrome (PRRSV) Type II (NA) \u2013 ORF7 gene v\u00e0 Type II high virulent (HP) \u2013 ORF1a gene<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">RNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">FAM: ORF7 gene of PRRSV-Type II (NA)<br \/>\nTexas Red: ORF1a gene of PRRSV-Type II-China (HP)<br \/>\nHEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects PRRSV-Type II (NA) v\u00e0 PRRSV-Type II-China (HP)<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\">PRRSV One-step RT-qPCR mix, negative control, positive control, internal control (IC), PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Caution<\/strong>:<\/p>\n<ul>\n<li>The detection of the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is most effective in pigs that are impacted during the early stages of PRRSV infection. This includes vulnerable piglets, lethargic pigs, weak nursing piglets, pigs exhibiting signs of anorexia, and recently farrowed sows\/sows with signs of fever, anorexia, and clinical symptoms.<\/li>\n<li>After sample collection, it is advisable to transport the samples under cold conditions and store them in a freezer until testing is conducted.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Extraction kit recommended for use: <\/strong><\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid Viral Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-dna-rna-co-extraction-kit-hi-004\/\">TopPURE\u00ae Serum Viral DNA\/RNA Co-Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> Detection and differentiation of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type II (North American lineage), characterized by the ORF7 gene, and the highly pathogenic Type II (HP), characterized by the ORF1a gene, associated with disease in pigs using One-step RT-qPCR.<\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n v\u00e0 ph\u00e2n bi\u1ec7t virus PRRSV Type II (NA) \u0111\u01b0\u1ee3c \u0111\u1eb7c tr\u01b0ng b\u1edfi gen ORF7 v\u00e0 Type II \u0111\u1ed9c l\u1ef1c cao (HP) b\u1edfi gen ORF1a g\u00e2y b\u1ec7nh tr\u00ean heo (l\u1ee3n) b\u1eb1ng k\u1ef9 thu\u1eadt One-step RT-qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7532","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7532"}],"version-history":[{"count":15,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7532\/revisions"}],"predecessor-version":[{"id":13951,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7532\/revisions\/13951"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7532"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7532"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7532"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}