{"id":7420,"date":"2022-10-13T17:10:49","date_gmt":"2022-10-13T09:40:49","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=7420"},"modified":"2025-08-08T12:27:19","modified_gmt":"2025-08-08T04:57:19","slug":"trisure-reagent-dd-030","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/trisure-reagent-dd-030\/","title":{"rendered":"TRISURE REAGENT"},"content":{"rendered":"<h2><strong>Introduction to TRISURE REAGENT<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p><strong>Trisure reagent<\/strong> is a ready-to-use, fully formulated solution designed for the simultaneous isolation of DNA, RNA, and protein from biological samples, including human, animal, plant, yeast, bacterial, and viral tissues.<\/p>\n<p>Upon application, <strong>Trisure reagent<\/strong>homogenizes and lyses the sample, resulting in phase separation into three distinct layers: a clear aqueous upper phase containing RNA, an interphase containing DNA, and a red organic lower phase containing protein.<\/p>\n<p>RNA is extracted from the aqueous phase via precipitation with 2-propanol. The <strong>Trisure reagent<\/strong> \u2019s potent RNase-inhibitory properties ensure the preservation of RNA integrity during lysis, facilitating the isolation of high-quality nucleic acids.<\/p>\n<p>DNA is recovered from the interphase by ethanol precipitation, while proteins are obtained through sequential precipitation from the phenol-ethanol supernatant using 2-propanol.<\/p>\n<p style=\"text-align: center;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-medium wp-image-13139\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-300x171.webp\" alt=\"trisure reagent\" width=\"300\" height=\"171\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-300x171.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-1024x583.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-768x438.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-1536x875.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp-1707x973.webp 1707w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/10\/trisure_webp.webp 1920w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<h2><strong>Manual instruction<\/strong><\/h2>\n<h4><\/h4>\n<h4><strong>1. Sample preparation<\/strong><\/h4>\n<p>- Tissue samples:<\/p>\n<ul>\n<li>Homogenize 50\u2013100\u202fmg of tissue in a 1.5\u202fmL microcentrifuge tube with 1\u202fmL of TRISURE Reagent.<\/li>\n<li>Note: Vortex intermittently during incubation.<\/li>\n<\/ul>\n<p>- Monolayer cell samples:<\/p>\n<ul>\n<li>Apply 1\u202fmL of TRISURE Reagent per 10\u202fcm<sup>2<\/sup> of glass culture dish surface.<\/li>\n<li>Note: Do not use TRISURE Reagent on plastic culture dishes.<\/li>\n<\/ul>\n<p>- Immune cells samples:<\/p>\n<ul>\n<li>Isolate cells by centrifugation, aspirate supernatant, add TRISURE Reagent, and mix thoroughly by pipetting.<\/li>\n<li>Use 1\u202fmL of reagent to lyse 10<sup>5<\/sup>\u00a0&#8211; 10<sup>6<\/sup> cells from animal, plant, yeast, or bacterial sources.<\/li>\n<\/ul>\n<h4><strong>2. Phase separation<\/strong><\/h4>\n<ul>\n<li>Step 1: Allow the sample to stand for 5 minutes at room temperature.<\/li>\n<li>Step 2: Add 0.1\u202fmL of 1-bromo-3-chloropropane or 0.2\u202fmL of chloroform to each 1\u202fmL of TRISURE Reagent.<\/li>\n<li>Step 3: Seal the tube, shake vigorously for 15 seconds, and incubate for 2\u201315 minutes at room temperature.<\/li>\n<li>Step 4: Centrifuge the mixture at 12,000\u00d7g for 15 minutes at 2\u20138\u00b0C.<\/li>\n<\/ul>\n<p>This centrifugation step separates the mixture into three distinct phases: upper aqueous phase (clear, contains RNA), interphase (contains DNA) and lower organic phase (red, contains protein).<\/p>\n<h4><strong>3. RNA isolation and extraction<\/strong><\/h4>\n<ul>\n<li>Step 1: Transfer the aqueous phase to a new tube. Add 0.5\u202fmL of 2-propanol per 1\u202fmL of TRISURE Reagent used during sample preparation. Mix thoroughly.<\/li>\n<li>Step 2: Incubate for 5\u201310 minutes at room temperature, then centrifuge at 12,000\u00d7g for 10 minutes at 2\u20138\u00b0C.<\/li>\n<li>Step 3: Discard the supernatant. Wash the RNA pellet by adding 1\u202fmL of 75% ethanol per 1\u202fmL of TRISURE Reagent used in sample preparation.<\/li>\n<li>Step 4: Vortex briefly to resuspend, then centrifuge at 7,500\u00d7g for 5 minutes at 2\u20138\u00b0C.<\/li>\n<li>Step 5: Rapidly dry the RNA pellet for 5\u201310 minutes at room temperature or under vacuum.<\/li>\n<li>Step 6: Add an appropriate volume of formamide, water, or 0.5% SDS solution to the RNA pellet. To facilitate dissolution, mix repeatedly by pipetting and incubate at 55\u201360\u00b0C for 10\u201315 minutes.<\/li>\n<\/ul>\n<h4 id=\"ftoc-heading-6\" class=\"ftwp-heading\"><strong>4. DNA isolation and extraction<\/strong><\/h4>\n<ul>\n<li>Step 1: Carefully remove any residual aqueous phase above the interphase. Add 0.3\u202fmL of 100% ethanol per 1\u202fmL of TRISURE Reagent used during sample preparation. Mix gently and incubate for 2\u20133 minutes at room temperature. Centrifuge at 2,000\u00d7g for 5 minutes at 2\u20138\u00b0C to precipitate DNA from the interphase and organic phase.<\/li>\n<li>Step 2: Wash the DNA pellet twice using a solution of 0.1\u202fM trisodium citrate in 10% ethanol. Use 1\u202fmL of wash solution per 1\u202fmL of TRISURE Reagent used. Each wash should last at least 30 minutes, with occasional gentle pipetting. Avoid disrupting the DNA pellet.<\/li>\n<li>Step 3: Centrifuge at 2,000\u00d7g for 5 minutes at 2\u20138\u00b0C. Store the DNA pellet in 1.5\u20132\u202fmL of 75% ethanol per 1\u202fmL of TRISURE Reagent used. Incubate for 10\u201320 minutes at room temperature.<\/li>\n<li>Step 4: Dry the DNA pellet under vacuum for 5\u201310 minutes. Dissolve the pellet in 8\u202fmM NaOH by pipetting.<\/li>\n<li>Step 5: Centrifuge at 12,000\u00d7g for 10 minutes to remove insoluble material. Transfer the supernatant to a new tube.<\/li>\n<\/ul>\n<h6 id=\"ftoc-heading-7\" class=\"ftwp-heading\">\u00a0 \u00a0<strong> \u00a0a. DNA Amplification by PCR<\/strong><\/h6>\n<p>After dissolving in 8\u202fmM NaOH, adjust the pH to 8.4 using HEPES free acid (add 86\u202f\u03bcL of 0.1\u202fM HEPES per mL of DNA solution). Add 0.1\u20131\u202f\u03bcg of DNA to the PCR mixture and proceed with standard amplification protocols<\/p>\n<h6><strong>\u00a0 \u00a0 \u00a0b. DNA Digestion with Restriction Enzymes<\/strong><\/h6>\n<p>Adjust the DNA solution to the optimal pH for enzyme activity using HEPES buffer, or dialyze against 1\u202fmM EDTA, pH 7\u20138. Incubate with restriction enzymes for 3\u201324 hours under optimal conditions.<\/p>\n<h4 id=\"ftoc-heading-6\" class=\"ftwp-heading\"><strong>5. Protein isolation<\/strong><\/h4>\n<ul>\n<li>Step 1: Precipitate proteins from the phenol\u2013ethanol supernatant (obtained during DNA isolation) by adding 1.5\u202fmL of 2-propanol per 1\u202fmL of TRISURE Reagent used during sample preparation. Allow the mixture to stand for at least 10 minutes at room temperature.\nCentrifuge at 12,000\u00d7g for 10 minutes at 2\u20138\u00b0C.<\/li>\n<li>Step 2: Discard the supernatant. Wash the protein pellet three times, each for 20 minutes, using a solution of 0.3\u202fM guanidine hydrochloride in 95% ethanol at room temperature. Use 2\u202fmL of wash solution per 1\u202fmL of TRISURE Reagent used during sample preparation.<\/li>\n<li>Step 3: Centrifuge at 7,500\u00d7g for 5 minutes at 2\u20138\u00b0C. After three washes, add 2\u202fmL of 100% ethanol, vortex briefly, and incubate for 20 minutes at room temperature. Centrifuge again at 7,500\u00d7g for 5 minutes at 2\u20138\u00b0C.<\/li>\n<li>Step 4: Dry the protein pellet under vacuum for 5\u201310 minutes. Resuspend the pellet in 1% SDS solution by pipetting. Centrifuge at 10,000\u00d7g for 10 minutes at 2\u20138\u00b0C to remove insoluble contaminants. Transfer the supernatant to a new tube. The protein solution should be used immediately for Western blotting or stored at \u201320\u00b0C for future use.<\/li>\n<\/ul>\n<p>Read more:<\/p>\n<ul>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product-category\/hoa-chat\/hoa-chat-shpt-co-ban\/\">Other Bio-reagents<\/a><\/li>\n<li><a class=\"connectButton\" href=\"https:\/\/drive.google.com\/drive\/u\/0\/folders\/1PrkTDPTW4sL0WUkUjRDkJUp0hTyXAvDi\" target=\"_blank\" rel=\"noopener\">Manual instruction<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> <strong>Trisure reagent<\/strong> is used to homogenize samples and inactivate RNases, enabling the isolation of DNA, RNA, and protein from human, animal, plant, yeast, bacterial, and viral tissue samples.<\/p>\n<p><strong>Storage:<\/strong> Store at 2-8\u00b0C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Packaging:<\/strong> 100mL bottle (Code: DD-030)<\/p>\n<p><strong>Manufacturer:<\/strong> ABT Vietnam<\/p>","protected":false},"featured_media":13139,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Trisure reagent \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u1ed3ng nh\u1ea5t m\u1eabu, b\u1ea5t ho\u1ea1t RNase, s\u1eed d\u1ee5ng ph\u00e2n l\u1eadp DNA\/RNA\/Protein t\u1eeb m\u1eabu m\u00f4 tr\u00ean ng\u01b0\u1eddi, \u0111\u1ed9ng v\u1eadt, th\u1ef1c v\u1eadt, n\u1ea5m men, vi khu\u1ea9n v\u00e0 vi r\u00fat. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: Nhi\u1ec7t \u0111\u1ed9 2-8\u00b0C H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t Quy c\u00e1ch: Chai&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7420","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7420"}],"version-history":[{"count":12,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7420\/revisions"}],"predecessor-version":[{"id":13145,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/7420\/revisions\/13145"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/13139"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7420"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=7420"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=7420"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=7420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}