{"id":6143,"date":"2022-05-20T08:00:07","date_gmt":"2022-05-20T08:00:07","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=6143"},"modified":"2025-11-27T10:18:46","modified_gmt":"2025-11-27T02:48:46","slug":"topspec-jev-rt-qpcr-kit-sqv-109","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-jev-rt-qpcr-kit-sqv-109\/","title":{"rendered":"TopSPEC\u00ae JEV RT-qPCR KIT (SQV-109)"},"content":{"rendered":"<h4><strong><strong>Introduction<\/strong> <span class=\"JsGRdQ\">TopSPEC\u00ae JEV RT-qPCR Kit<\/span><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\">Japanese Encephalitis (JE) is not only a disease affecting humans but also occurs in various mammalian species, particularly livestock such as pigs, cattle, and buffaloes. Following viral entry into the body, it infiltrates the bloodstream and migrates to various organs. Ultimately, during the developmental stage, the JEV causes damage to the liver, kidneys, spleen, muscles, and, ultimately, the central nervous system. Infected hosts typically exhibit compromised immune systems. In particular, pregnant individuals infected with the virus may experience a significantly high rate of miscarriages. The disease is transmissible at any age, highlighting the importance of early detection as the most effective preventive measure for livestock breeders.<\/p>\n<p><span class=\"JsGRdQ\"><strong>TopSPEC\u00ae JEV RT-qPCR Kit<\/strong> is a diagnostic assay designed to detect the presence of Japanese encephalitis virus (JEV) in pigs, cattle, horses, buffaloes, and other livestock using One-step RT-qPCR technology targeting the NS2A gene. Early identification of the pathogen helps reduce economic losses associated with JEV infection.<\/span><\/p>\n<picture><img fetchpriority=\"high\" decoding=\"async\" class=\"lazyautosizes lazyloaded wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae JEV One-step RT-qPCR Kit\" width=\"300\" height=\"168\" data-eio=\"p\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-300x168.jpeg.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-1024x575.jpeg.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-768x431.jpeg.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1-18x10.jpeg.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/02\/PCR-CHUA-CHIA-KHONG-SAN-IC-1536x862-1.jpeg.webp 1536w\" data-sizes=\"auto\" data-eio-rwidth=\"300\" data-eio-rheight=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/picture>\n<p style=\"text-align: center;\"><span class=\"JsGRdQ\"><strong>TopSPEC\u00ae JEV RT-qPCR Kit<\/strong><\/span><\/p>\n<p><strong><strong>Highlights<\/strong><\/strong><\/p>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time was optimized for 2 hours<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<p><strong><strong>Specification<\/strong><\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"414\"><em>Japanese encephalitis<\/em> (JEV) \u2013 NS2A gene<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"414\">Serum sample and whole blood sample<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"414\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>RNA volume<\/strong><\/td>\n<td width=\"414\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"414\">FAM: NS2A gene of JEV<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"414\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"414\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"414\">Kit only detects JEV<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"414\">JEV RT-qPCR Mix, Negative control, Positive control, Internal control (IC), PCR tube<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"414\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Viral DNA\/RNA Extraction Kit<\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> A diagnostic kit for detecting Japanese encephalitis virus (JEV), the causative agent of Japanese encephalitis in livestock (pigs, cattle, buffaloes, etc.), using One-step RT-qPCR technology targeting the NS2A gene.<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: B\u1ed9 kit ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n JEV - gene NS2A g\u00e2y b\u1ec7nh vi\u00eam n\u00e3o Nh\u1eadt B\u1ea3n tr\u00ean gia s\u00fac (heo, b\u00f2, tr\u00e2u,\u2026) b\u1eb1ng k\u1ef9 thu\u1eadt One-step RT-qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=6143"}],"version-history":[{"count":13,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6143\/revisions"}],"predecessor-version":[{"id":13948,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6143\/revisions\/13948"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=6143"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=6143"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=6143"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=6143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}