{"id":6123,"date":"2022-05-18T09:24:25","date_gmt":"2022-05-18T09:24:25","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=6123"},"modified":"2025-10-09T16:19:59","modified_gmt":"2025-10-09T08:49:59","slug":"topspec-vnn-rt-qpcr-kit-sqf-212","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-vnn-rt-qpcr-kit-sqf-212\/","title":{"rendered":"TopSPEC\u00ae VNN One-step RT-qPCR KIT (SQF-212)"},"content":{"rendered":"<h4><strong>INTRODUCTION<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p>Viral Nervous Necrosis <em>(VNN<\/em>) typically occurs in fish aged 10\u201345 days. Infected fish appear lethargic and swim near the water surface. Fish older than 45 days may exhibit uncoordinated swimming in circular or spiral patterns. In addition, fish with VNN often show reduced appetite, and body parts may darken. Mortality rates reach 70\u2013100% in larvae and juveniles, and around 20% in market-size fish. Therefore, early screening and monitoring during the fry stage are crucial to minimize economic losses for farmers.<\/p>\n<p class=\"translation-block\"><strong>TopSPEC\u00ae VNN One-step RT-qPCR Kit<\/strong> is a test kit that detects the presence of virus<em>\u00a0<\/em>that causes neuronecrosis (VNN) in the culture environment . Early detection of pathogens helps save lost costs caused by VNN.<\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong>TopSPEC\u00ae VNN One-step RT-qPCR Kit<\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time was optimized for 2 hours<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"348\"><em>(VNN<\/em> ) \u2013 coat protein gen<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"348\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">RNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"348\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>RNA volume<\/strong><\/td>\n<td width=\"348\">\u00a05 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"348\">FAM: detect VNN<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"348\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"348\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"348\">Only VNN is detected<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"348\">VNN One-step RT-qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube.<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"348\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-tissua-viral-extraction-kit-hi-312\/\">TopPURE\u00ae Tissue viral extraction kit\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit <\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose:<\/strong> kit to detect the causative agent of neuronecrosis disease (VNN) in fish using Real-time PCR technique<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>\n<p>&nbsp;<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: b\u1ed9 kit ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed th\u1ea7n kinh (VNN) tr\u00ean c\u00e1 b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam) 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