{"id":6117,"date":"2022-05-18T09:15:31","date_gmt":"2022-05-18T09:15:31","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=6117"},"modified":"2025-10-09T11:06:21","modified_gmt":"2025-10-09T03:36:21","slug":"topspec-mrnv-rt-qpcr-kit-sqs-252","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-mrnv-rt-qpcr-kit-sqs-252\/","title":{"rendered":"TopSPEC\u00ae MrNV One-step RT-qPCR KIT (SQS-252)"},"content":{"rendered":"<h2><strong>Introducing TopSPEC\u00ae MrNV One-step\u00a0 RT-qPCR Kit<\/strong><\/h2>\n<p><em>White Tail Disease (WTD)<\/em> or<em> White Muscle Disease (WMD)<\/em> occurs in giant freshwater prawns and is caused by <em>Macrobrachium rosenbergii nodavirus<\/em> (MrNV) and <em>extra small virus<\/em> (XSV). MrNV is the primary causative agent of the disease, while XSV also plays a significant role. MrNV infection does not cause mortality in adult shrimp; however, it can be transmitted from broodstock to larvae and juveniles, leading to death. The mortality rate among larvae and juvenile shrimp reaches 100% within 5\u20136 days after the onset of the disease. Therefore, early screening and detection of the MrNV virus should be prioritized to minimize losses.<\/p>\n<p class=\"translation-block\"><strong>TopSPEC\u00ae MrNV One-step\u00a0 RT-qPCR Kit<\/strong> is a test kit that detects the presence of <em>Macrobrachium rosenbergii nodavirus<\/em> (MrNV) in the culture environment. Early detection of pathogens helps save costs caused by MrNV.<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><em>The <strong>TopSPEC\u00ae MrNV One-step RT-qPCR Kit <\/strong>(undivided master mix)<\/em><\/p>\n<h2><strong><strong>Highlights<\/strong> <\/strong><\/h2>\n<ul>\n<li>Simple procedure<\/li>\n<li>PCR time was optimized for 2 hours<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h2><strong><strong>Specification<\/strong><\/strong><\/h2>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"348\"><em>Macrobrachium <\/em><em>R<\/em><em>osenbergii <\/em><em>N<\/em><em>odavirus<\/em> (MrNV) &#8211; RNA dependent RNA Polymerase<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"348\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">RNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"348\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>RNA volume<\/strong><\/td>\n<td width=\"348\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"348\">FAM: detect MrNV<br \/>\nHEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"348\">TaqMan probe\/One-step RT-qPCR<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"348\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"348\">Only MrNV is detected<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"348\">\u00a0MrNV One-step RT-qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube.<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"348\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-highpure-viral-extraction-kit-exs-012\/\">TopPURE\u00ae Highpure Viral Extraction Kit (EXS-021)<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit (HI-612)<\/a><\/p>\n<p><strong>Read more<\/strong>: <a href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o&amp;t=38s\" target=\"_blank\" rel=\"noopener\">Instructional video for the qualitative Real-time PCR kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose<\/strong>: kit to detect MrNV agent causing white tail and muscle disease on shrimp using Real-time PCR technique<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: b\u1ed9 kit ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n MrNV g\u00e2y b\u1ec7nh tr\u1eafng \u0111u\u00f4i v\u00e0 c\u01a1 tr\u00ean t\u00f4m b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6117","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=6117"}],"version-history":[{"count":13,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6117\/revisions"}],"predecessor-version":[{"id":13569,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6117\/revisions\/13569"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=6117"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=6117"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=6117"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=6117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}