{"id":6113,"date":"2022-05-18T08:52:10","date_gmt":"2022-05-18T08:52:10","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=6113"},"modified":"2025-10-09T11:39:06","modified_gmt":"2025-10-09T04:09:06","slug":"topspec-nhpb-qpcr-kit-sqs-172","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-nhpb-qpcr-kit-sqs-172\/","title":{"rendered":"TopSPEC\u00ae NHPB qPCR KIT (SQS-172)"},"content":{"rendered":"<h4><strong>Introduction<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\"><strong>Necrotizing hepatopancreatitis<\/strong>, also known as black spot disease, is caused by <em>Necrotizing hepatopancreatitis bacterium<\/em> (NHPB). The disease occurs when the pond environment, especially the bottom of the pond, is of poor quality. The mortality rate is up to 95% within 15-30 days of getting sick. The infected shrimps show signs of lethargy, loss of appetite, slow growth, having tiny black spots or large patches and other symptoms. Without timely preventive measures, the situation will get worse. Early detection is the best measure.<\/p>\n<p><strong>TopSPEC\u00ae NHPB qPCR Kit<\/strong> is a testing kit designed to detect the presence of <em>Necrotizing <\/em><em>H<\/em><em>epatopancreatitis <\/em><em>B<\/em><em>acterium<\/em> (NHPB) in aquaculture environments. Early detection of the pathogen helps minimize economic losses caused by NHPB.<\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-11787 size-medium aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong>TopSPEC\u00ae NHPB qPCR Kit<\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong> <\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"348\"><em>Necrotizing <\/em><em>H<\/em><em>epatopancreatitis <\/em><em>B<\/em><em>acterium<\/em> (NHPB) &#8211; 16S ribosomal RNA gene<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"348\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">DNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"348\">200 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"348\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"348\">FAM: detect NHPB<\/p>\n<p>HEX: internal control<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"348\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"348\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"348\">Only NHPB is detected<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"348\">NHPB qPCR mix, negative control, positive control, internal control(IC), Tube PCR.<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"348\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-highpure-viral-extraction-kit-exs-012\/\">TopPURE\u00ae Highpure Viral Extraction Kit (EXS-021)<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit (HI-612)<\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"translation-block\"><strong>Purpose:<\/strong> The kit detects the agent <em>Necrotizing hepatopancreatitis bacterium<\/em> (NHPB) that causes liver necrosis disease in shrimp using Real-time PCR technique.<\/p>\n<p><strong>Storage:<\/strong> -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: b\u1ed9 kit ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n Necrotizing hepatopancreatitis bacterium (NHPB) g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed gan tr\u00ean t\u00f4m b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6113","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=6113"}],"version-history":[{"count":15,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6113\/revisions"}],"predecessor-version":[{"id":13573,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6113\/revisions\/13573"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=6113"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=6113"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=6113"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=6113"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}