{"id":6002,"date":"2022-05-05T09:17:14","date_gmt":"2022-05-05T09:17:14","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=6002"},"modified":"2026-03-02T12:06:58","modified_gmt":"2026-03-02T04:36:58","slug":"toppure-ffpe-dna-extraction-kit-hi-182","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/toppure-ffpe-dna-extraction-kit-hi-182\/","title":{"rendered":"TopPURE\u00ae FFPE DNA EXTRACTION KIT CE (HI-182)"},"content":{"rendered":"<h2><strong>INTRODUCTION<\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p class=\"translation-block\">FFPE is a cancer biopsy tissue sample, fixed with formalin and molded paraffin to help preserve tissue structure intact for a long time. This is also a great resource for retrospective research on cancer, histopathology, and molecular genetics. Currently, gene sequencing, Realtime PCR are often used to accurately detect gene mutations, quantify cancer gene expression, etc. Therefore, DNA extraction from FFPE tissue is an important first step.<\/p>\n<p><strong>TopPURE\u00ae FFPE DNA Extraction Kit<\/strong> \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t d\u1ef1a tr\u00ean c\u00f4ng ngh\u1ec7 c\u1ed9t silica theo ti\u00eau chu\u1ea9n ISO 9001 v\u00e0 ISO 13485 cho ph\u00e9p thu nh\u1eadn <strong>DNA<\/strong>\u00a0ch\u1ea5t l\u01b0\u1ee3ng cao.<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"translation-block\"><strong>HIGHLIGHTS<\/strong><\/h2>\n<ul>\n<li>Safety - Solvents are not toxic<\/li>\n<li>The quality of the resulting DNA has a high integrity, helping to increase PCR amplification of large segments<\/li>\n<li>Follow to silica column principle: easy, convenient<\/li>\n<li>Efficiency: able to collect 12ug<\/li>\n<li>Completed solution: include Paraffin removal step and DNA extraction<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2><strong><strong>SPECIFICATIONS<\/strong> <\/strong><\/h2>\n<ul>\n<li>Sample type: FFPE<\/li>\n<li>Input sample volume: 8 samples with 5-10\u00b5m thick<\/li>\n<li>Efficiency: up to 12\u00b5g<\/li>\n<li>Extraction time: <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">3 hour for 10 samples<\/span><\/li>\n<li>Purity:<span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">OD260\/280 ~ 1.7-2.2<\/span><\/li>\n<li class=\"translation-block\">Storage: Buffers can be stored dry at room temperature. Proteinase K and RNA Carrier are stored at 2-8<sup>o<\/sup>C<\/li>\n<li>Expiration date: 24 months from the manufacturing date<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2><strong>COMPONENTS<\/strong><\/h2>\n<table width=\"372\">\n<tbody>\n<tr>\n<td width=\"120\"><strong>Component<\/strong><\/td>\n<td width=\"252\"><strong>Storage<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"120\">Mineral oil<\/td>\n<td rowspan=\"9\" width=\"252\" class=\"translation-block\">Room temperature<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">TL Buffer<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">CL buffer<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">WB1 Buffer<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">WB2 Buffer<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">EB Buffer<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">Ethanol<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">Silica column<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">Tube 1.5 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"120\">Proteinase K<\/td>\n<td width=\"252\">2-8<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4><\/h4>\n<h2><strong>DATA EXPERIMENT\u00a0<\/strong><\/h2>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-6003\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2022\/05\/Picture2-300x148.png\" alt=\"TopPURE\u00ae FFPE DNA Extraction Kit\" width=\"636\" height=\"314\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2022\/05\/Picture2-300x148.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/05\/Picture2-64x31.png 64w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/05\/Picture2-18x9.png 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2022\/05\/Picture2.png 624w\" sizes=\"(max-width: 636px) 100vw, 636px\" \/><\/p>\n<p><strong>Figure 1 <\/strong>PCR results of extracted products for <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">\u03b2<\/span>actin gene with varying fragment sizes<\/p>\n<h2><\/h2>\n<h2><strong>Read more:<\/strong><\/h2>\n<p><a href=\"https:\/\/abtvn.com\/en\/product-category\/kit-tach-chiet\/tach-chiet-cot\/\">Other column extraction kit<\/a><\/p>\n<p><a href=\"https:\/\/www.youtube.com\/@ABTBIOLOGICALSOLUTIONSCOLTD\/videos\" target=\"_blank\" rel=\"noopener\">Instructional Video for ABT Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong> <strong>TopPURE\u00ae FFPE DNA Extraction Kit <\/strong>Used for DNA extraction from cancer biopsy tissue samples, formalin fixation and paraffin casting.<\/p>\n<p><strong>Storage:<\/strong> <strong>Storage:<\/strong> Spin columns and buffers can be stored dry at room temperature. Proteinase K is stored at 2-8<sup>o<\/sup>C)<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":7066,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: TopPURE\u00ae FFPE DNA Extraction Kit \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho t\u00e1ch chi\u1ebft DNA t\u1eeb m\u1eabu m\u00f4 sinh thi\u1ebft ung th\u01b0, c\u1ed1 \u0111\u1ecbnh b\u1eb1ng formalin v\u00e0 \u0111\u00fac parafin. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (ri\u00eang proteinase K l\u01b0u tr\u1eef \u1edf nhi\u1ec7t \u0111\u1ed9 2-8oC) Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6002","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=6002"}],"version-history":[{"count":16,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6002\/revisions"}],"predecessor-version":[{"id":14418,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/6002\/revisions\/14418"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/7066"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=6002"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=6002"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=6002"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=6002"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}