{"id":14365,"date":"2026-02-05T08:59:18","date_gmt":"2026-02-05T01:29:18","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=14365"},"modified":"2026-02-05T09:10:12","modified_gmt":"2026-02-05T01:40:12","slug":"topspec-pdd-multiplex-qpcr-kit-mqs-203","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-pdd-multiplex-qpcr-kit-mqs-203\/","title":{"rendered":"TopSPEC\u00ae PDD multiplex qPCR Kit (MQS-203)"},"content":{"rendered":"<h4><strong>About TopSPEC\u00ae PDD multiplex qPCR Kit<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Photobacterium damselae subsp. damselae<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> is a marine bacterium belonging to the family Vibrionaceae. It is Gram-negative, rod-shaped, and motile. Colonies of PDD appear opaque white on BHI agar and green on TCBS medium. Together with <em>Photobacterium damselae subsp. piscicida<\/em> , PDD represents one of the two principal subspecies of <\/span><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Photobacterium damselae<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">.PDD is recognized as a highly virulent pathogen, causing septicemia in numerous marine fish species, often associated with high mortality rates and significant economic losses in aquaculture.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">In addition to fish, PDD has been reported to cause severe disease in shrimp, with extremely high mortality rates documented in countries such as China, Thailand, India, and Japan. Infected shrimp typically exhibit anorexia, abnormal swimming behavior, lethargy, hepatopancreatic atrophy, pale intestines, soft body texture, and notably, a distinct discoloration of the body ranging from pallor to a milky-white appearance.<\/span><\/p>\n<p>Historically, PDD had not been detected or reported in shrimp in Vietnam. Previous studies and reports only documented PDD infections in marine fish species (e.g., cobia, grouper) across the northern, central, and southern regions. However, recent findings have confirmed the presence of PDD in whiteleg shrimp (Litopenaeus vannamei) through 16S rRNA gene sequencing and comparison with published PDD sequences available in genetic databases.<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC \u00ae PDD multiplex qPCR Kit<\/span><\/strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> is a diagnostic assay specifically developed to detect the presence of the haemolysin A and damselysin toxin genes, as well as the urease enzyme gene, in <\/span><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">P. damselae ssp. Damselae<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> (PDD), a pathogen responsible for shrimp diseases, using Real-time PCR technology.<\/span><\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-11787 size-medium\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC \u00ae KHV qPCR Kit\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC \u00ae PDD multiplex qPCR Kit<\/span><\/strong><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<table style=\"height: 499px;\" width=\"1121\">\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Genes encoding the haemolysin A toxin (hlyApl* gene), damselysin (dly gene), and the urease enzyme (ureC gene) in <em>P. damselae ssp. Damselae<\/em> (PDD)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"414\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"414\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"414\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: hlApl gene (haemolysin A)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Texas Red: dly gene (damselysin)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Cy5: ureC gene (urease)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"414\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"414\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">The kit is designed solely for the detection and\ndifferentiation of PDD<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">PDD multiplex qPCR Mix, Negative control, PDD\nmultiplex Positive control, Internal control (IC),\nTube PCR<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"414\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Recommended extraction kit:<\/strong><\/p>\n<ul>\n<li>Silica column extraction:\u00a0<a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Fluid Viral Extraction Kit<\/span><\/a><\/li>\n<li>Manual magnetic bead extraction: <a style=\"font-size: 16px; background-color: #ffffff;\" href=\"https:\/\/abtvn.com\/en\/product\/kit-tach-chiet-dna-rna-thu-cong\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Manual Maga DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li>Automated magnetic bead extraction: <a style=\"font-size: 16px; background-color: #ffffff;\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit\u00a0<\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>TopSPEC\u00ae PDD multiplex qPCR Kit is designed to detect the presence of the haemolysin A and damselysin toxin genes, as well as the urease enzyme gene, in <em>P. damselae ssp. Damselae <\/em>(PDD), a pathogen responsible for shrimp diseases, using Real-time PCR technology.<\/p>\n<p><strong>Storage<\/strong>: -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: TopSPEC \u00ae PDD multiplex qPCR Kit d\u00f9ng \u0111\u1ec3 ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a gene \u0111\u1ed9c t\u1ed1 haemolysin A, damselysin v\u00e0 enzyme urease \u1edf vi khu\u1ea9n P. damselae ssp. Damselae (PDD) g\u00e2y b\u1ec7nh tr\u00ean t\u00f4m b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng:&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14365","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=14365"}],"version-history":[{"count":2,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14365\/revisions"}],"predecessor-version":[{"id":14367,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14365\/revisions\/14367"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=14365"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=14365"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=14365"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=14365"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}