{"id":14127,"date":"2025-12-24T17:59:28","date_gmt":"2025-12-24T10:29:28","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=14127"},"modified":"2026-03-09T08:46:20","modified_gmt":"2026-03-09T01:16:20","slug":"topspec-asfv-pedv-rt-qpcr-kit-mqv-126","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-asfv-pedv-rt-qpcr-kit-mqv-126\/","title":{"rendered":"TopSPEC\u00ae ASFV\/PEDV RT-qPCR Kit (MQV-126)"},"content":{"rendered":"<h3><strong>About TopSPEC\u00ae ASFV\/PEDV RT-qPCR<span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">\u00a0Kit<\/span><\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">African Swine Fever Virus (ASFV) <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">and<\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> Porcine Epidemic Diarrhea Virus (PEDV) <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">are two highly infectious agents that pose serious threats to global livestock production, causing severe economic losses.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">ASFV is a DNA virus belonging to the family <\/span><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Asfarviridae<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">, genus <\/span><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Asfivirus<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> and is associated with mortality rates of up to 100% across all age groups of pigs. In contrast, PEDV is a positive single-stranded RNA (+ssRNA) virus classified within the family <\/span><em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Coronavirus<\/span><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">, genus <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><em>Alphacoronavirus<\/em>.<\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> PEDV infection leads to vomiting, diarrhea, dehydration, anorexia, and weight loss. The disease is particularly severe in neonatal piglets, with mortality rates reaching 100% when infected with highly virulent strains.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Both viruses spread rapidly through direct contact, contaminated feed, or inadequately disinfected transport vehicles. Early detection is therefore critical for effective control and prevention of outbreaks.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae ASFV\/PEDV RT-qPCR Kit<\/span><\/strong> <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">enables simultaneous detection of African Swine Fever Virus (ASFV) via the Vp72 gene and Porcine Epidemic Diarrhea Virus (PEDV) via the N gene, using a one-step RT-qPCR technique. Early identification of these pathogens is essential to minimize economic losses caused by viral infections.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-11787 size-medium\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC \u00ae Anaplasma\/ Babesia Multiplex qPCR Kit\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC \u00ae ASFV\/PEDV RT-qPCR<span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">\u00a0Kit<\/span><\/span><\/strong><\/p>\n<h3><strong><strong>Highlights<\/strong><\/strong><\/h3>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimized PCR time was approximately 2 hours<\/li>\n<li>High specificity and sensitivity.<\/li>\n<\/ul>\n<h3><strong><strong>Specification<\/strong><\/strong><\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">ASFV - Vp72 gene and PEDV - N gene<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">DNA\/RNA after extraction<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: Vp72 gene of ASFV<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Texas Red: N gene of PEDV<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: Internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">1 hour 45 minutes<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Kit only detects ASFV and PEDV<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<p>Silica column extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit-he-001\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Genomic DNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-extraction-kit-he-002\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Fluid Viral Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-stool-dna-extraction-kit-he-006\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Stool DNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<p>Automatic magnetic beads extraction:<\/p>\n<ul>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-quick-viral-extration-kit-hi-812-2\/\">TopPURE\u00ae Maga Viral DNA\/RNA Extraction Kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-dna-rna-extraction-kit-hm-001-a\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Purpose: The TopSPEC\u00ae ASFV\/PEDV RT\u2011qPCR Kit<\/strong> is a diagnostic kit designed to simultaneously detect African Swine Fever Virus (ASFV) through the Vp72 gene and Porcine Epidemic Diarrhea Virus (PEDV) through the N gene, using the One\u2011step RT\u2011qPCR technique.<\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: TopSPEC \u00ae ASFV\/PEDV RT-qPCR\u00a0Kit l\u00e0 b\u1ed9 kit x\u00e9t nghi\u1ec7m gi\u00fap ki\u1ec3m tra \u0111\u1ed3ng th\u1eddi s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a virus d\u1ecbch t\u1ea3 heo ch\u00e2u Phi (ASFV) th\u00f4ng qua gene Vp72 v\u00e0 virus ti\u00eau ch\u1ea3y c\u1ea5p \u1edf heo (PEDV) th\u00f4ng qua gene N, s\u1eed d\u1ee5ng k\u1ef9 thu\u1eadt One-step RT-qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14127","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=14127"}],"version-history":[{"count":3,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14127\/revisions"}],"predecessor-version":[{"id":14472,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/14127\/revisions\/14472"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=14127"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=14127"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=14127"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=14127"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}