{"id":13984,"date":"2025-11-27T18:05:34","date_gmt":"2025-11-27T10:35:34","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=13984"},"modified":"2025-11-27T18:11:56","modified_gmt":"2025-11-27T10:41:56","slug":"topspec-tilv-rt-qpcr-kit-sqf-104","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-tilv-rt-qpcr-kit-sqf-104\/","title":{"rendered":"TopSPEC\u00ae TiLV RT-qPCR Kit (SQF-104)"},"content":{"rendered":"<h4><strong>About TopSPEC\u00ae TiLV RT-qPCR Kit<\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><em><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Tilapia Lake Virus (TiLV<\/span><\/strong><\/em><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><em><strong>)<\/strong><\/em> is an infectious pathogen that causes disease across all life stages of both farmed and wild tilapia. Clinical signs are generally nonspecific and include lethargy, anorexia, ocular alterations (such as corneal opacity, exophthalmia, and cloudiness), dermatological changes (erosions, hemorrhages, and darkened pigmentation), abdominal distension\/ascites, and scale loss.<\/span> <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TiLV can be detected in multiple organs, including the gills, spleen, anterior kidney, liver, brain, and heart. Transmission occurs both horizontally (via contact, mucus, feces, and water) and vertically (from broodstock to eggs or milt).<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae TiLV RT-qPCR <\/span><\/strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Kit<\/strong> is designed to detect the presence of Tilapia Lake Virus by targeting the hypothetical protein gene, which causes disease in tilapia, using RT-qPCR technology.<\/span><\/p>\n<h4><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-11787 size-medium\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae TiLV RT-qPCR Kit\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/h4>\n<p style=\"text-align: center;\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae TiLV RT-qPCR <\/span><\/strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Kit<\/strong> <\/span><\/p>\n<h4><strong><strong>Highlights<\/strong><\/strong><\/h4>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<h4><strong><strong>Specification<\/strong><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<table style=\"height: 499px;\" width=\"1121\">\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Tilapia Lake Virus \u2013 Hypothetical protein gene<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"414\">RNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"414\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"414\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: TiLV<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"414\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"414\">1 hour 45 minutes<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Kit only detects TiLV<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"414\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TiLV RT-qPCR Mix, Negative control, Positive control, Internal control (IC), PCR tube<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"414\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-viral-dna-rna-extraction-kit-he-008\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\" style=\"font-size: 16px;\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE \u00ae Viral DNA\/RNA Extraction Kit<\/span><\/span><\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-rna-extraction-kit-hi-212\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Serum Viral RNA Extraction Kit<\/span><\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-dna-rna-extraction-kit-hm-001-a\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga DNA\/RNA Extraction Kit<\/span> <\/a><\/p>\n<p><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-serum-dna-rna-co-extraction-kit-hi-007\/\">TopPURE\u00ae Maga Serum DNA\/RNA Co-Extraction Kit<\/a><\/p>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong><span class=\"WdYUQQ\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae TiLV RT-qPCR <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Kit <\/span><\/strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is designed to detect the presence of Tilapia Lake Virus by targeting the hypothetical protein gene, which causes disease in tilapia, using RT-qPCR technology.<\/span><\/span><\/p>\n<p><strong>Storage<\/strong>: -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch:TopSPEC\u00ae TiLV RT-qPCR Kit d\u00f9ng \u0111\u1ec3 ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a Tilapia Lake Virus \u2013 Hypothetical protein gene \u2013 g\u00e2y b\u1ec7nh tr\u00ean c\u00e1 r\u00f4 phi b\u1eb1ng k\u1ef9 thu\u1eadt RT-qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13984","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=13984"}],"version-history":[{"count":1,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13984\/revisions"}],"predecessor-version":[{"id":13985,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13984\/revisions\/13985"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=13984"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=13984"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=13984"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=13984"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}