{"id":13310,"date":"2025-09-10T09:52:00","date_gmt":"2025-09-10T02:22:00","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=13310"},"modified":"2025-10-07T09:14:27","modified_gmt":"2025-10-07T01:44:27","slug":"topspec-asfv-mgf-qpcr-kit-sqv-017","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-asfv-mgf-qpcr-kit-sqv-017\/","title":{"rendered":"TopSPEC\u00ae ASFV-MGF qPCR Kit (SQV-017)"},"content":{"rendered":"<h3><strong>About TopSPEC\u00ae <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">ASFV-MGF<\/span>\u00a0qPCR KIT<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><em>African swine fever virus<\/em> (ASFV) <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is a highly pathogenic agent that poses a significant threat to the swine industry. Among its genetic components, the Multigene Family (MGF) genes encode proteins that suppress type I interferon responses, enabling the virus to evade the host immune system and replicate efficiently. These MGF genes are divided into two major regions: the right variable region (RVR) and the left variable region (LVR). Wild-type ASFV strains typically retain intact MGF regions and exhibit high virulence, whereas certain attenuated vaccine strains show deletions in one of these regions (RVR or LVR), resulting in reduced pathogenicity.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae ASFV-MGF qPCR Kit<\/span><\/strong> <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is a diagnostic tool designed to detect the presence of <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">MGF gene<\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> regions (RVR and LVR) in ASFV strains infecting pigs, using Real-time PCR technology.<\/span><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-11787 size-medium\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"ASFV\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC \u00ae <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">ASFV-MGF<\/span>\u00a0<strong>qPCR Kit<\/strong><\/span><\/strong><\/p>\n<h3><strong><strong>Highlights<\/strong><\/strong><\/h3>\n<ul>\n<li>Simple procedure<\/li>\n<li>The optimized PCR duration is approximately 1.5 hours<\/li>\n<li>High specificity and sensitivity.<\/li>\n<\/ul>\n<h3><strong><strong>Specification<\/strong><\/strong><\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">ASFV \u2013 MGF gene regions (RVR and LVR)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Quantitative detection<\/strong><\/td>\n<td width=\"425\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: MGF360-9L (LVR)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: MGF360-12L (RVR)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Cy5: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">1.5h<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Kit only detects the MGF gene regions of ASFV<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<p>Silica column extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit-he-001\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE \u00ae Genomic DNA Extraction Kit<\/span><\/a><\/li>\n<li><a style=\"font-size: 16px; background-color: #ffffff;\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-blood-dna-extraction-kit-he-007\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Blood DNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\" style=\"font-size: 16px;\">TopPURE \u00ae Fluid Viral Extraction Kit<\/span><\/a><\/li>\n<li><span style=\"font-size: 16px;\"><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-serum-viral-dna-rna-co-extraction-kit-hi-004\/\"><span style=\"font-size: 16px;\">TopPURE\u00ae Serum Viral DNA\/RNA Co-Extraction Kit<\/span><\/a><br \/>\n<\/span><\/li>\n<\/ul>\n<p>Automatic magnetic beads extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-manual-maga-viral-dna-rna-kit-hi-009\/\"><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE \u00ae Manual Maga Viral DNA\/RNA Kit<\/span><\/a><\/li>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-serum-dna-rna-co-extraction-kit-hi-007\/\">TopPURE\u00ae Maga Serum DNA\/RNA Co-Extraction Kit<\/a><\/li>\n<li><a class=\"connectButton\" href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-quick-viral-extration-kit-hi-812-2\/\">TopPURE\u00ae Maga Viral DNA\/RNA Extraction Kit<\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae ASFV-MGF qPCR Kit <\/span><\/strong><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is a diagnostic tool designed to detect the presence of <\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">MGF gene<\/span><span class=\"a_GcMg font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> regions (RVR and LVR) in ASFV strains infecting pigs, using Real-time PCR technology.<\/span><\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: TopSPEC\u00ae ASFV-MGF qPCR Kit l\u00e0 b\u1ed9 kit x\u00e9t nghi\u1ec7m gi\u00fap ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a v\u00f9ng MGF gene (RVR v\u00e0 LVR) \u1edf ASFV g\u00e2y b\u1ec7nh tr\u00ean heo b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13310","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=13310"}],"version-history":[{"count":2,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13310\/revisions"}],"predecessor-version":[{"id":13312,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/13310\/revisions\/13312"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=13310"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=13310"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=13310"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=13310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}