{"id":12258,"date":"2025-02-26T14:54:13","date_gmt":"2025-02-26T07:24:13","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=12258"},"modified":"2025-10-07T09:49:35","modified_gmt":"2025-10-07T02:19:35","slug":"topspec-isospora-suis-qpcr-kit-sqv-014","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-isospora-suis-qpcr-kit-sqv-014\/","title":{"rendered":"TopSPEC\u00ae Isospora suis qPCR KIT (SQV-014)"},"content":{"rendered":"<h3><strong>About TopSPEC\u00ae Isospora suis qPCR KIT<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\">Swine coccidiosis is caused by the parasite <em>Isospora suis<\/em>, affecting pigs of all ages but most commonly observed in neonatal piglets under two weeks old. The disease is prevalent in high-density pig farming areas with poor sanitation conditions. Its characteristic symptoms include enteritis and diarrhea, which may appear as liquid or pasty stool\u2014initially milky white and later turning yellow with a foul odor. Infected piglets may exhibit weakness, dehydration, stunted growth, reduced weight gain, and, in severe cases, death.<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\"><strong>TopSPEC\u00ae Isospora suis qPCR Kit<\/strong> is a diagnostic kit designed to detect the presence of <em>Isospora suis<\/em> \u2013 ITS1 gene \u2013 associated with coccidiosis in pigs using the qPCR technique. The kit utilizes a master mix containing UNG (Uracil-N-Glycosylase), which helps prevent cross-contamination from previous PCR products.<\/p>\n<p style=\"text-align: center;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-medium wp-image-11787\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae Isospora suis qPCR Kit\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae Isospora suis qPCR Kit<\/span><\/strong><\/p>\n<h3><strong><strong>Highlights<\/strong><\/strong><\/h3>\n<ul>\n<li>Simple procedure<\/li>\n<li>The optimized PCR duration is approximately 1.5 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h3><strong><strong>Specification<\/strong><\/strong><\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><em>Isospora suis<\/em> &#8211; ITS1 gene<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"425\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: <em>Isospora suis<\/em><\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: internal control<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects<em> <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Isospora suis<\/span><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">.<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><em>Isospora suis<\/em> qPCR mix, negative control, positive control, internal control (IC), PCR tube<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<p>Silica column extraction:<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid viral extraction kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Genomic DNA extraction kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-stool-dna-extraction-kit-he-006\/\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Stool DNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>Automatic magnetic beads extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-quick-viral-extration-kit-hi-812-2\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Viral DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-manual-maga-viral-dna-rna-kit-hi-009\/\">TopPURE\u00ae Manual Maga Viral DNA\/RNA Kit<\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>Detection\u00a0<span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">the presence of <strong><em>Isospora suis &#8211; <\/em><\/strong>\u00a0<strong><em>ITS1 gene<\/em><\/strong> associated with coccidiosis in pigs using the qPCR technique.<\/span><\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra\u00a0s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a Isospora suis - \u00a0ITS1 gene g\u00e2y b\u1ec7nh c\u1ea7u tr\u00f9ng tr\u00ean heo (l\u1ee3n) b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT Vietnam","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12258","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=12258"}],"version-history":[{"count":4,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12258\/revisions"}],"predecessor-version":[{"id":13488,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12258\/revisions\/13488"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=12258"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=12258"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=12258"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=12258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}