{"id":12244,"date":"2025-02-25T15:16:42","date_gmt":"2025-02-25T07:46:42","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=12244"},"modified":"2025-10-07T10:15:43","modified_gmt":"2025-10-07T02:45:43","slug":"topspec-pasteurella-multocida-qpcr-kit-sqv-010","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-pasteurella-multocida-qpcr-kit-sqv-010\/","title":{"rendered":"TopSPEC\u00ae Pasteurella multocida qPCR KIT (SQV-010)"},"content":{"rendered":"<h3><strong>About TopSPEC\u00ae <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Pasteurella multocida <\/span> qPCR KIT<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\"><em>Pasteurella multocida<\/em> is a Gram-negative bacterium belonging to the family <em>Pasteurellaceae<\/em>. It is the causative agent of hemorrhagic septicemia (HS), a highly fatal disease in pigs. Hemorrhagic septicemia can affect pigs of all ages but is most commonly observed in those between 3 and 6 months old. The pathogen primarily resides in the respiratory mucosa, and under adverse conditions\u2014such as environmental changes or herd relocation\u2014pigs may experience weakened immunity, allowing the bacteria to proliferate and cause disease.<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae Pasteurella multocida qPCR Kit<\/span><\/strong> <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is a testing kit designed to detect the presence of <\/span><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Pasteurella multocida<\/span><\/em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> - KMT1 gene \u2013 associated with hemorrhagic septicemia in pigs using the qPCR technique. The kit utilizes a master mix containing UNG (Uracil-N-Glycosylase), which helps prevent cross-contamination from previous PCR products.<\/span><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/images.fineartamerica.com\/images-medium-large-5\/pasteurella-multocida-bacteria-steve-gschmeissnerscience-photo-library.jpg\" alt=\"Pasteurella Multocida Bacteria by Science Photo Library\" width=\"575\" height=\"493\" \/><\/p>\n<p style=\"text-align: center;\">Illustration of <em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Pasteurella multocida<\/span><\/em>\u00a0bacteria causing disease in pigs.<\/p>\n<h3><strong><strong>Highlights<\/strong><\/strong><\/h3>\n<ul>\n<li>Simple procedure<\/li>\n<li>The optimized PCR duration is approximately 1.5 hours<\/li>\n<li>High specificity and sensitivity<\/li>\n<li>Taqman probe technology<\/li>\n<\/ul>\n<h3><strong><strong>Specification<\/strong><\/strong><\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"425\"><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Pasteurella multocida<\/span><\/em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"> &#8211; KMT1 gene<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample<\/strong><\/td>\n<td width=\"425\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Input sample volume<\/strong><\/td>\n<td width=\"425\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"425\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: KMT1 gene of <em>Pasteurella multocida <\/em><\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: internal control<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Flourescent channel<\/strong><\/td>\n<td width=\"425\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"425\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"425\">Kit only detects<em> <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Pasteurella multocida<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"425\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Kit components<\/strong><\/td>\n<td width=\"425\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">PM qPCR mix, negative control, PM positive control, internal control (IC), PCR tube<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>Extraction kit recommended for use:<\/strong><\/p>\n<p>Silica column extraction:<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Fluid viral extraction kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-fluid-viral-dna-rna-extraction-kit\/\">TopPURE\u00ae Genomic DNA extraction kit<\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-blood-dna-extraction-kit-he-007\/\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopPURE\u00ae Blood DNA Extraction Kit<\/span><\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>Automatic magnetic beads extraction:<\/p>\n<ul>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-quick-viral-extration-kit-hi-812-2\/\"><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">TopPURE\u00ae Maga Viral DNA\/RNA Extraction Kit<\/span><\/a><\/li>\n<li><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-manual-maga-viral-dna-rna-kit-hi-009\/\">TopPURE\u00ae Manual Maga Viral DNA\/RNA Kit<\/a><\/li>\n<\/ul>\n<p>Read more:\u00a0<a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses: <\/strong>Detection\u00a0<span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">the presence of<em> Pasteurella multocida<\/em> \u2013 KMT1 gene \u2013 associated with porcine hemorrhagic septicemia using the qPCR technique.<\/span><\/p>\n<p><strong>Storage:<\/strong> Store at -20<sup>o<\/sup>C<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer<\/strong>: ABT Vietnam<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: Ki\u1ec3m tra\u00a0s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a Pasteurella multocida - KMT1 gene - g\u00e2y b\u1ec7nh t\u1ee5 huy\u1ebft tr\u00f9ng tr\u00ean heo (l\u1ee3n) b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: B\u1ea3o qu\u1ea3n \u1edf -20oC H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT Vietnam","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12244","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=12244"}],"version-history":[{"count":3,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12244\/revisions"}],"predecessor-version":[{"id":13494,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12244\/revisions\/13494"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=12244"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=12244"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=12244"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=12244"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}