{"id":12062,"date":"2025-02-07T16:03:27","date_gmt":"2025-02-07T08:33:27","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=12062"},"modified":"2025-10-06T12:29:27","modified_gmt":"2025-10-06T04:59:27","slug":"topspec-gentamicin-mic-test-lak-105","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-gentamicin-mic-test-lak-105\/","title":{"rendered":"TopSPEC\u00ae Gentamicin MIC Test (LAK-105)"},"content":{"rendered":"<h2 class=\"cvGsUA direction-ltr align-start para-style-body\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">About TopSPEC\u00ae Gentamicin MIC Test<\/span><\/strong><\/h2>\n<p class=\"cvGsUA direction-ltr align-start para-style-body translation-block\">Minimum Inhibitory Concentration (MIC) is the lowest concentration of an antibacterial substance that visibly inhibits the growth of microorganisms. MIC assists in selecting the most appropriate antibiotics, preventing antibiotic misuse, contributing to the reduction of bacterial antibiotic resistance, shortening treatment duration, saving costs, and enhancing treatment effectiveness.<\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae Gentamicin MIC Test<\/strong> is a diagnostic kit designed to determine the Minimum Inhibitory Concentration (MIC)\u2014the lowest concentration of antibiotic capable of inhibiting bacterial growth.<\/span><\/p>\n<h3 class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Product description<\/span><\/h3>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Purpose of Use: To determine the Minimum Inhibitory Concentration (MIC)\u2014the lowest concentration of antibiotic capable of inhibiting bacterial growth.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Components: Gentamicin antibiotic plates, 50X R staining reagent, Sterile water<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Packaging: 16 tests<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Product code: LAK-105<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Storage Conditions: 2\u20138\u202f\u00b0C<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<h3 class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Procedure<\/span><\/h3>\n<p><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Step 1: Preparation<\/span><\/strong><\/p>\n<p><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Bacterial Sample: Culture bacterial colonies on non-selective agar plates for 18\u201324 hours. Prepare a bacterial suspension with a turbidity equivalent to McFarland 0.5.<\/span><\/p>\n<p><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- 50X R Staining Reagent:<\/span><\/p>\n<ul>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Spindown before manipulation<\/span><\/li>\n<li>Add the staining solution to sterile water at a ratio of 1:50<\/li>\n<li>Amount of solution used: 300\u00b5L\/1 strip (1 test)<\/li>\n<\/ul>\n<p><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Culture medium: Use CAMHB for easily cultured aerobic strains or LHB-CAMHB for fastidious or microaerophilic strains. (Note: Medium is not included in the kit.)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Step 2: Execution<\/span><\/strong><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Prepare approximately 3\u202fmL of medium per test<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Add 100\u202f\u00b5L of medium to the NEG wells<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Dilute the McFarland 0.5 bacterial suspension at a ratio of 1:300 in the culture medium<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Inoculate 100\u202f\u00b5L of the bacterial suspension into all wells except the NEG wells<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">- Incubate at 35\u201337\u202f\u00b0C for 16 to 20 hours<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-9971 aligncenter\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/Gen-300x145.jpeg\" alt=\"TopSPEC\u00ae Gentamicin MIC Test\" width=\"389\" height=\"188\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/Gen-300x145.jpeg 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/Gen-18x9.jpeg 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/Gen.jpeg 479w\" sizes=\"(max-width: 389px) 100vw, 389px\" \/><\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\" style=\"text-align: center;\"><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Figure 1: Layout of Gentamicin Concentration Levels on a Single Plate (Concentration Unit: \u03bcg\/mL)<\/span><\/em><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">*Note:<\/span><\/p>\n<ul>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">NEG: Well containing only the medium<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">POS: Well containing only the bacterial suspension<\/span><\/li>\n<\/ul>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Results reading day:<\/span><\/p>\n<ul>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Add 25\u03bcL of staining solution R (diluted 1:50 in sterile water) to all wells<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Incubate the plate for 30-40 minutes at 35-37\u00b0C<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Read the MIC results.<\/span><\/li>\n<\/ul>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Step 3<\/span><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">: Results reading<\/span><\/strong><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><img decoding=\"async\" class=\"alignnone wp-image-9945 aligncenter lazyload\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/hinh-kq-mic-300x103.jpeg\" alt=\"TopSPEC\u00ae Ampicillin MIC Test\" width=\"565\" height=\"194\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/hinh-kq-mic-300x103.jpeg 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/hinh-kq-mic-768x263.jpeg 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/hinh-kq-mic-18x6.jpeg 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/hinh-kq-mic.jpeg 960w\" data-sizes=\"(max-width: 565px) 100vw, 565px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 565px; --smush-placeholder-aspect-ratio: 565\/194;\" \/><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\" style=\"text-align: center;\"><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Figure 2: Representative outcomes following MIC testing.<\/span><\/em><\/p>\n<ul>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Negative control wells (NEG) with no color change (still showing the green color of the staining solution) ensure that the culture medium is not contaminated.<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Positive control wells (POS) exhibit a color change (pink or light pink), ensuring the stability of the staining solution and the normal growth of bacteria.<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Wells showing a color change from green to pink or no color change after adding the staining solution indicate the biochemical reactions of live bacteria, generating chemical reactions with the staining solution, resulting in a color change. This demonstrates the presence of viable bacteria.<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">The well containing the lowest antibiotic concentration that shows no color change (retains a blue hue) after the addition of the staining reagent is considered the MIC value\u2014the minimum inhibitory concentration capable of preventing bacterial growth. This lack of color change indicates insufficient viable bacteria to trigger the chemical reactions responsible for color transformation, thereby confirming the antibiotic\u2019s inhibitory effect at that concentration.<\/span><\/li>\n<li><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">In cases where the color changes from green to purple (or not entirely pink), the MIC value is approximately between this antibiotic concentration and the higher neighboring concentration.<\/span><\/li>\n<\/ul>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Read more:<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><a class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-underline text-strikethrough-none\" draggable=\"false\" href=\"https:\/\/abtvn.com\/en\/product-category\/kit-mic\/\" target=\"_blank\" rel=\"noopener\">Other MIC Test Kit<\/a><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><a class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-underline text-strikethrough-none\" draggable=\"false\" href=\"https:\/\/www.youtube.com\/@ABTBIOLOGICALSOLUTIONSCOLTD\" target=\"_blank\" rel=\"noopener\">Instructional Video for ABT Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Intended uses:<\/strong> <strong>TopSPEC\u00ae Gentamicin MIC Test<\/strong> is a diagnostic kit designed to determine the Minimum Inhibitory Concentration (MIC)\u2014the lowest concentration of antibiotic capable of inhibiting bacterial growth.<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Storage:<\/strong> 2 \u2013 8\u00b0C<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Package:<\/strong> 16 tests<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-start para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\"><strong>Code:<\/strong> LAK-105<\/span><\/p>","protected":false},"featured_media":9961,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[527,526],"product_tag":[],"class_list":["post-12062","product","type-product","status-publish","has-post-thumbnail","product_cat-kit-mic","product_cat-kit-chan-doan-khac","desktop-align-center","tablet-align-center","mobile-align-center","ast-product-gallery-layout-horizontal","ast-product-tabs-layout-distributed","first","instock","shipping-taxable","product-type-simple"],"uagb_featured_image_src":{"full":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105.jpg",1920,1077,false],"thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-150x150.jpg",150,150,true],"medium":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-300x168.jpg",300,168,true],"medium_large":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-768x431.jpg",768,431,true],"large":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-1024x574.jpg",1024,574,true],"1536x1536":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-1536x862.jpg",1536,862,true],"2048x2048":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105.jpg",1920,1077,false],"trp-custom-language-flag":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-18x10.jpg",18,10,true],"woocommerce_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-1707x958.jpg",1707,958,true],"woocommerce_single":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105.jpg",1920,1077,false],"woocommerce_gallery_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/03\/LAK-105-100x100.jpg",100,100,true]},"uagb_author_info":{"display_name":"ABT Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: TopSPEC\u00ae Gentamicin MIC Test l\u00e0 b\u1ed9 kit x\u00e9t nghi\u1ec7m gi\u00fap x\u00e1c \u0111\u1ecbnh gi\u00e1 tr\u1ecb MIC (Minimum Inhibitory Concentration) \u2013 n\u1ed3ng \u0111\u1ed9 kh\u00e1ng sinh th\u1ea5p nh\u1ea5t c\u00f3 th\u1ec3 ng\u0103n ch\u1eb7n s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a vi khu\u1ea9n. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: 2 \u2013 8\u00b0C Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 16 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12062","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=12062"}],"version-history":[{"count":4,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12062\/revisions"}],"predecessor-version":[{"id":13464,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/12062\/revisions\/13464"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/9961"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=12062"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=12062"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=12062"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=12062"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}