{"id":11981,"date":"2025-01-21T19:44:53","date_gmt":"2025-01-21T12:14:53","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&#038;p=11981"},"modified":"2025-10-11T10:47:14","modified_gmt":"2025-10-11T03:17:14","slug":"topspec-e-coli-o157-qpcr-kit-sqb-111","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-e-coli-o157-qpcr-kit-sqb-111\/","title":{"rendered":"TopSPEC\u00ae E.coli O157 qPCR KIT (SQB-111)"},"content":{"rendered":"<h4><strong><strong>Introduction<\/strong> <span class=\"OYPEnA text-decoration-none text-strikethrough-none\"><strong>TopSPEC\u00ae<\/strong> <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">E.coli O157<\/span><\/span><span class=\"OYPEnA text-decoration-none text-strikethrough-none\">\u00a0<strong>qPCR Kit<\/strong><\/span><\/strong><\/h4>\n<p>&nbsp;<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body translation-block\"><em>Escherichia coli<\/em> O157 is a Gram-negative, aerobic, rod-shaped bacterium with a cell wall structure containing a thin layer of peptidoglycan. The bacteria have pili that enable them to adhere to surfaces, particularly the intestinal mucosa. They also possess an outer membrane containing lipopolysaccharides, which play a crucial role in toxicity. <em>E. coli <\/em>O157 is recognized as a major cause of severe gastrointestinal illnesses in humans, such as bloody diarrhea and hemolytic uremic syndrome (HUS). It is commonly transmitted through contact or consumption of contaminated food, particularly undercooked beef, untreated water, and unpasteurized milk.<\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae E.coli O157 qPCR Kit<\/span><\/strong> <span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">is a screening kit used to detect the presence of <em>Escherichia coli<\/em> O157 tr\u00ean m\u1eabu th\u1ef1c ph\u1ea9m b\u1eb1ng k\u1ef9 thu\u1eadt qPCR d\u1ef1a tr\u00ean s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a gen <\/span><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">rfbE.<\/span><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-11787 size-medium\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp\" alt=\"TopSPEC\u00ae E.coli O157 qPCR Kit\" width=\"300\" height=\"168\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-300x168.webp 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1024x575.webp 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-768x431.webp 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1536x862.webp 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-2048x1150.webp 2048w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-18x10.webp 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/11\/PCR-CHUA-CHIA-NO-IC-1707x958.webp 1707w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">TopSPEC\u00ae E.coli O157 qPCR Kit<\/span><\/strong><\/p>\n<p><strong><strong>Highlights<\/strong><\/strong><\/p>\n<ul>\n<li>Simple procedure<\/li>\n<li>Optimize the PCR time to 1.5 hours.<\/li>\n<li>Various input sample types<\/li>\n<li>High specificity and sensitivity<\/li>\n<\/ul>\n<p><strong><strong>Specification<\/strong><\/strong><\/p>\n<p>&nbsp;<\/p>\n<table style=\"height: 499px;\" width=\"1121\">\n<tbody>\n<tr>\n<td width=\"174\"><strong>Targets<\/strong><\/td>\n<td width=\"414\"><em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Escherichia coli O157<\/span><\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Sample types<\/strong><\/td>\n<td width=\"414\">DNA after extraction<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>DNA volume<\/strong><\/td>\n<td width=\"414\">5 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Color channel detection<\/strong><\/td>\n<td width=\"414\">\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">FAM: detect <em>Escherichia coli<\/em> O157<\/span><\/p>\n<p class=\"cvGsUA direction-ltr align-justify para-style-body\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">HEX: internal control (IC)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Technology <\/strong><\/td>\n<td width=\"414\">TaqMan probe<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>PCR time<\/strong><\/td>\n<td width=\"414\">1.5h<\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Specificity<\/strong><\/td>\n<td width=\"414\">Kit only detects <em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Escherichia coli <\/span><\/em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">O157<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Component<\/strong><\/td>\n<td width=\"414\"><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">E.coli O157 <\/span><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">qPCR mix, negative control, positive control, internal control, PCR tube<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"174\"><strong>Storage<\/strong><\/td>\n<td width=\"414\">12 months at -20<sup>o<\/sup>C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><strong>Recommended extraction kit<\/strong> <\/strong><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-genomic-dna-extraction-kit\/\">TopPURE\u00ae Genomic DNA Extraction Kit\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-food-dna-extraction-kit-he-013\/\">TopPURE\u00ae Food DNA Extraction Kit\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/abtvn.com\/en\/product\/toppure-maga-genomic-dna-rna-extraction-kit\/\">TopPURE\u00ae Maga Genomic DNA\/RNA Extraction Kit\u00a0<\/a><\/p>\n<p>Read more: <a class=\"connectButton\" href=\"https:\/\/www.youtube.com\/watch?v=AlfN42DIe0o\" target=\"_blank\" rel=\"noopener\">Instructional Video for Qualitative Real-time PCR Kit<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p><strong>Intended uses:<\/strong><span class=\"WdYUQQ\"> Check for the presence of <em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">Escherichia coli <\/span><\/em><span class=\"OYPEnA font-feature-liga-off font-feature-clig-off font-feature-calt-off text-decoration-none text-strikethrough-none\">O157 (<em>E.coli<\/em> O157) <\/span><\/span><span class=\"WdYUQQ\">\u00a0in food samples by real-time PCR<\/span><\/p>\n<p><strong>Storage<\/strong>: -20<sup>o<\/sup>C<\/p>\n<p><strong>Package:<\/strong> 50 tests<\/p>\n<p class=\"translation-block\"><strong>Expiration date<\/strong>: 12 months from the date of manufacture<\/p>\n<p><strong>Manufacturer:<\/strong> ABT (Vietnam)<\/p>","protected":false},"featured_media":11787,"comment_status":"open","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/"},"uagb_comment_info":0,"uagb_excerpt":"M\u1ee5c \u0111\u00edch: s\u00e0ng l\u1ecdc vi khu\u1ea9n Escherichia coli O157 (E.coli O157) \u00a0trong vi sinh th\u1ef1c ph\u1ea9m b\u1eb1ng k\u1ef9 thu\u1eadt qPCR. \u0110i\u1ec1u ki\u1ec7n l\u01b0u tr\u1eef: -20oC Quy c\u00e1ch \u0111\u00f3ng g\u00f3i: 50 tests H\u1ea1n s\u1eed d\u1ee5ng: 12 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t H\u00e3ng: ABT (Vi\u1ec7t Nam)","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/11981","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=11981"}],"version-history":[{"count":5,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/11981\/revisions"}],"predecessor-version":[{"id":13694,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product\/11981\/revisions\/13694"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11787"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=11981"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_brand?post=11981"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_cat?post=11981"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/product_tag?post=11981"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}