{"id":11768,"date":"2024-12-26T16:27:30","date_gmt":"2024-12-26T08:57:30","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&p=11768"},"modified":"2025-10-11T16:17:16","modified_gmt":"2025-10-11T08:47:16","slug":"abt-urine-prep-solution-hi-010","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/abt-urine-prep-solution-hi-010\/","title":{"rendered":"ABT\u00ae Urine Prep Solution (HI-010)"},"content":{"rendered":"

Overview\u00a0<\/strong><\/h3>\n

Previously, the urinary tract was considered a sterile environment, except in cases of urinary tract infection. However, recent studies using culture and molecular biology methods have shown that urine contains a relatively small amount of bacteria (less than 10^5 CFU\/mL of urine), much lower than the average of 10^12 CFU\/mL found in other parts of the body such as the intestines and the vagina. Nevertheless, urine is a reliable non-invasive sample commonly used to detect sexually transmitted bacteria or parasites such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium<\/em>, and Trichomonas vaginalis<\/em> through nucleic acid amplification methods. Additionally, evaluating the microbiota present in the urinary tract plays an important role in clinical screening, which is still being widely researched, such as for urethritis, urinary tract infections, and bladder cancer. Furthermore, urine collection is more acceptable to men compared to urethral swab collection. However, optimal methods for processing urine samples for PCR-based research have not been widely studied.<\/p>\n

To address the issue of low bacterial concentration in urine, increasing urine volume (>30 mL) is an approach commonly used to enhance DNA recovery yield. However, urine typically contains high concentrations of PCR inhibitors such as urea, \u03b2 HCG, and salt crystals that interfere with bacterial DNA detection. Urine is often refrigerated or frozen to prevent bacterial overgrowth and nucleic acid degradation. However, storing urine at low temperatures can lead to the precipitation of certain crystals such as calcium oxalate, uric acid, and amorphous phosphate or urate crystals, which can reduce DNA yield and inhibit molecular biology applications. Centrifuging large volumes of urine can exacerbate the problem of DNA extraction and PCR inhibition. Heating urine to dissolve these crystals is one approach, but it can also lead to bacterial cell membrane lysis, DNA degradation, or uncontrolled bacterial proliferation. Besides temperature, pH is also a factor in the formation of crystals in urine. For example, uric acid and amorphous urate crystals tend to form in acidic urine (usually pH <5.8), while calcium phosphate and amorphous phosphate crystals form in neutral or alkaline urine. Some crystals, like calcium oxalate, can form regardless of pH with sufficient concentrations of Ca2+<\/sup> in the urine.<\/p>\n

Products ABT \u00ae Urine Prep Solution<\/strong> provides a urine processing solution containing components that dissolve salt crystals and stabilize the DNA and microorganisms present in the urine. This enhances DNA extraction efficiency and improves target detection in PCR assays compared with untreated urine.<\/p>\n

ABT \u00ae Urine Prep Solution<\/strong><\/h3>\n

Intended use:<\/strong> Processing urine samples to remove salts and impurities for DNA\/RNA extraction purposes.<\/p>\n

Specification:<\/strong><\/p>\n