{"id":11726,"date":"2024-12-09T11:53:21","date_gmt":"2024-12-09T04:23:21","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&p=11726"},"modified":"2025-10-09T09:39:21","modified_gmt":"2025-10-09T02:09:21","slug":"topspec-v-parahaemolyticus-ehp-pcr-kit-sps-002","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-v-parahaemolyticus-ehp-pcr-kit-sps-002\/","title":{"rendered":"TopSPEC\u00ae V.parahaemolyticus\/EHP PCR Kit (SPS-002)"},"content":{"rendered":"

About TopSPEC\u00ae V.parahaemolyticus\/EHP PCR Kit<\/strong><\/h4>\n

 <\/p>\n

Early Mortality Syndrome (EMS) and microsporidiosis are significant challenges in shrimp aquaculture.<\/span><\/p>\n

EMS, or Acute Hepatopancreatic Necrosis Disease (AHPND), is induced by Vibrio parahaemolyticus, which secretes toxins that impair the hepatopancreas, resulting in high mortality rates within shrimp populations.<\/p>\n

– <\/span>Microsporidiosis<\/span><\/em> caused by a parasite <\/span>Enterocytozoon Hepatopenaei <\/span>(EHP)<\/span><\/em> Shrimp infected with microsporidiosis often exhibit slow growth because the parasite lives in the hepatopancreas and absorbs the shrimp\u2019s nutrients. The disease does not cause mass mortality but still leads to significant economic losses due to reduced production.<\/span><\/p>\n

TopSPEC\u00ae V.parahaemolyticus\/EHP PCR KIT<\/span><\/strong><\/em> is a diagnostic kit designed to detect the presence of EHP and <\/span>V. paraheamolyticus<\/span><\/em> in shrimp samples using PCR technology.\nThe kit offers outstanding advantages such as rapid testing time, high accuracy, a simple procedure, and the ability to detect multiple pathogens within a single amplification reaction.<\/span><\/p>\n

\"\"<\/h4>\n

TopSPEC\u00ae V.parahaemolyticus\/EHP PCR KIT<\/span><\/strong><\/em><\/p>\n

Specification<\/strong><\/strong><\/h4>\n\n\n\n\n\n\n\n\n\n\n
Targets<\/strong><\/td>\nVibrio parahaemolyticus – EHP<\/span><\/td>\n<\/tr>\n
Input sample<\/strong><\/td>\nGills, swimming legs, stool samples from shrimp<\/span><\/td>\n<\/tr>\n
Input sample volume<\/strong><\/td>\n200 \u00b5L<\/td>\n<\/tr>\n
DNA volume<\/strong><\/td>\n5 \u00b5L<\/td>\n<\/tr>\n
PCR time<\/strong><\/td>\n2 hours \u2013 2 hours 30 minutes (depending on the model)<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nKit only detects Vibrio parahaemolyticus v\u00e0 EHP<\/span><\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n12 months at -20o<\/sup>C<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\nVE PCR Mix, negative control, positive control<\/span>, tube PCR<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Extraction kit recommended for use: <\/strong>TopPURE \u00ae Highpure Viral extraction kit (EXS-021)<\/a><\/p>\n

Recommended electrophoresis kits: <\/strong>TopSPEC\u00ae Agarose Electrophoresis (AEP-01)<\/a><\/p>\n

Result analysis<\/strong><\/p>\n

\u2013 After electrophoresis is completed, the results are visualized on a UV transilluminator or a gel documentation system equipped with UV light.<\/p>\n

\u2013 100bp DNA ladder used includes 10 lines from 100 to 1000 bp<\/p>\n

\u2013 Expected results are as follows:<\/p>\n